Holstege J C, Van Dijken H, Buijs R M, Goedknegt H, Gosens T, Bongers C M
Department of Anatomy, Erasmus University Medical School, Rotterdam, The Netherlands.
J Comp Neurol. 1996 Dec 23;376(4):631-52. doi: 10.1002/(SICI)1096-9861(19961223)376:4<631::AID-CNE10>3.0.CO;2-P.
In the present study, the distribution of dopamine (DA) was identified light microscopically in all segments of the rat, cat, and monkey spinal cord by using immunocytochemistry with antibodies directed against dopamine. Only fibers and (presumed) terminals were found to be immunoreactive for DA. Strongest DA labeling was present in the sympathetic intermediolateral cell column (IML). Strong DA labeling, consisting of many varicose fibers, was found in all laminae of the dorsal horn, including the central canal area (region X), but with the exception of the substantia gelatinosa, which was only sparsely labeled, especially in rat and monkey. In the motoneuronal cell groups DA labeling was also strong and showed a fine granular appearance. The sexually dimorphic cremaster nucleus and Onuf's nucleus (or its homologue) showed a much stronger labeling than the surrounding somatic motoneurons. In the parasympathetic area at sacral levels, labeling was moderate. The remaining areas, like the intermediate zone (laminae VI-VIII), were only sparsely innervated. The dorsal nucleus (column of Clarke) showed the fewest DA fibers, as did the central cervical nucleus, suggesting that cerebellar projecting cells were avoided by the DA projection. In all species, the descending fibers were located mostly in the dorsolateral funiculus, but laminae I and III also contained many rostrocaudally oriented fibers. It is concluded that DA is widely distributed within the spinal cord, with few differences between species, emphasizing that DA plays an important role as one of the monoamines that influences sensory input as well as autonomic and motor output at the spinal level.
在本研究中，通过使用针对多巴胺的抗体进行免疫细胞化学，在光镜下确定了多巴胺（DA）在大鼠、猫和猴脊髓所有节段中的分布。仅发现纤维和（推测的）终末对DA具有免疫反应性。最强的DA标记出现在交感中间外侧细胞柱（IML）中。在背角的所有层，包括中央管区域（X区），都发现了由许多曲张纤维组成的强烈DA标记，但胶状质除外，其标记稀疏，尤其是在大鼠和猴中。在运动神经元细胞群中，DA标记也很强，并呈现出细颗粒状外观。具有性别差异的提睾肌核和奥努夫核（或其同源物）的标记比周围的躯体运动神经元强得多。在骶段的副交感神经区域，标记为中等强度。其余区域，如中间带（VI - VIII层），仅接受稀疏的神经支配。背核（克拉克柱）和颈中央核中的DA纤维最少，这表明DA投射避开了向小脑投射的细胞。在所有物种中，下行纤维大多位于背外侧索，但I层和III层也含有许多 rostrocaudally 定向的纤维。得出的结论是，DA在脊髓内广泛分布，物种间差异很小，这强调了DA作为一种单胺在影响脊髓水平的感觉输入以及自主和运动输出方面发挥着重要作用。
