Ryan K, Garrett N, Mitchell A, Gurdon J B
Department of Zoology, University of Cambridge, England.
Cell. 1996 Dec 13;87(6):989-1000. doi: 10.1016/s0092-8674(00)81794-8.
Eomesodermin (Eomes) is a novel Xenopus T-domain gene. In normal development, it is expressed in mesodermal cells in a ventral-to-dorsal gradient of increasing concentration. It reaches its peak expression 1-2 hr before any other known panmesodermal gene. It is strongly inducible by normal vegetal cells and by mesoderm-inducing factors. Ectopic expression of Eomes in animal caps induces the transcription of nearly all mesodermal genes in a concentration-dependent way. Overexpression of Eomes dorsalizes ventral mesoderm, inducing gsc and changing cell fate to muscle and notochord. Blocking the function of Eomes causes gastrulation arrest and defective mesoderm-dependent gene activation. We propose that Eomes fulfills an essential function in initiating mesoderm differentiation and in determining mesodermal cell fate.
胚中胚层决定蛋白（Eomes）是一种新的非洲爪蟾T结构域基因。在正常发育过程中，它在中胚层细胞中以浓度从腹侧到背侧递增的梯度表达。在任何其他已知的全中胚层基因之前1-2小时，它达到表达峰值。它可被正常植物细胞和中胚层诱导因子强烈诱导。Eomes在动物帽中的异位表达以浓度依赖的方式诱导几乎所有中胚层基因的转录。Eomes的过表达使腹侧中胚层背化，诱导 goosecoid（gsc）表达并将细胞命运转变为肌肉和脊索。阻断Eomes的功能会导致原肠胚形成停滞和中胚层依赖性基因激活缺陷。我们提出，Eomes在启动中胚层分化和确定中胚层细胞命运方面发挥着重要作用。
