Márquez C, Egozcue J, Martorell M R, Moreno V, Templado C
Departamento de Biología Celular, Facultad de Medicina, Universidad Autónoma de Barcelona.
Cytogenet Cell Genet. 1996;72(2-3):164-70. doi: 10.1159/000134177.
The effect of Colcemid on the frequency and type of chromosomal abnormalities in human sperm was investigated. Using the human sperm and zona-free hamster egg fusion technique, penetrated eggs were cultured in the presence or absence of Colcemid. We used two different times of Colcemid treatment: standard Colcemid treatment (Colcemid-5 h) or long Colcemid treatment (Colcemid-17 h). Each Colcemid series had its own control series without Colcemid, thus ensuring that Colcemid was the only significant variable. A total of 771 sperm karyotypes from one normal donor was analyzed: 286 in the Colcemid-5 h series, 262 in the Colcemid-17 h series, and 223 in the two control series. In both Colcemid series there was a significant increase in the frequencies of hypohaploidy vs. hyperhaploidy (9.4% and 7.3% vs. 2.4% and 1.1%, for the Colcemid-5 h and Colcemid-17 h series, respectively), in contrast to those obtained in the control series, in which the frequencies of hypohaploidy and hyperhaploidy were close to the 1:1 relationship (4.9% vs. 4.0%) expected from nondisjunction. There was a significant increase in the frequency of structural abnormalities in both Colcemid series (16.1% and 14.5% for the Colcemid-5 h and Colcemid-17 h series, respectively) compared to the control series (6.3%). These results suggest that Colcemid significantly increases the frequency of hypohaploidy and unstable structural aberrations in human sperm.
研究了秋水仙酰胺对人类精子染色体异常频率和类型的影响。采用人类精子与无透明带仓鼠卵融合技术，将穿透的卵子在有或无秋水仙酰胺的情况下进行培养。我们使用了两种不同的秋水仙酰胺处理时间：标准秋水仙酰胺处理（秋水仙酰胺-5小时）或长时间秋水仙酰胺处理（秋水仙酰胺-17小时）。每个秋水仙酰胺系列都有其自身不含秋水仙酰胺的对照系列，从而确保秋水仙酰胺是唯一的显著变量。对来自一名正常供体的771个精子核型进行了分析：秋水仙酰胺-5小时系列中有286个，秋水仙酰胺-17小时系列中有262个，两个对照系列中有223个。在两个秋水仙酰胺系列中，亚单倍体与超单倍体的频率均显著增加（秋水仙酰胺-5小时系列分别为9.4%和7.3%，秋水仙酰胺-17小时系列分别为2.4%和1.1%），这与对照系列的结果形成对比，在对照系列中亚单倍体和超单倍体的频率接近非分离预期的1:1关系（4.9%对4.0%）。与对照系列（6.3%）相比，两个秋水仙酰胺系列中结构异常的频率均显著增加（秋水仙酰胺-5小时系列和秋水仙酰胺-17小时系列分别为16.1%和14.5%）。这些结果表明，秋水仙酰胺显著增加了人类精子中亚单倍体和不稳定结构畸变的频率。
