Rahman S M, Itakura H
Department of Pathology, Institute of Tropical Medicine, Nagasaki University, Japan.
Anal Quant Cytol Histol. 1996 Dec;18(6):471-80.
To investigate the value of morphometry in comparative histologic analysis of diseases, fixing a set of parameters suitable for analysis and to create a general strategy for data storage and economical data management.
To establish the following fundamental principles for morphometric measurements: (1) linear measurements to determine the distance between two points by using an eyepiece graticule with an engraved scale or alternatively with a ruler on a projected microscopic image or photograph; (2) Stereology based on geometric probability, allowing features in three dimensions to be measured from two-dimensional images; and (3) a computerized image processing and analysis system for image capture, storage and analysis using specialized software and hardware.
Measurement procedures were demonstrated in different parameters using regenerative nodules of alcoholic cirrhosis as a model. Comparative analysis between primary biliary cirrhosis, alcoholic cirrhosis, posthepatitic cirrhosis and hepatocellular carcinoma showed the highest regenerative activity in posthepatitic cirrhosis.
With current advances in computer technology, rapid, automatic measurements can be made from tissue sections and a variety of practical applications for the pathology laboratory, such as nuclear morphometry for comparison of different kinds of liver diseases, including classification of hepatocellular carcinoma.
研究形态计量学在疾病比较组织学分析中的价值，确定一套适合分析的参数，并创建数据存储和经济数据管理的总体策略。
为形态计量测量确立以下基本原则：（1）线性测量，通过使用带有刻度的目镜测微尺，或者在投射的显微镜图像或照片上使用尺子来确定两点之间的距离；（2）基于几何概率的体视学，允许从二维图像测量三维特征；（3）使用专门的软件和硬件进行图像捕获、存储和分析的计算机图像处理与分析系统。
以酒精性肝硬化的再生结节为模型，在不同参数中展示了测量程序。原发性胆汁性肝硬化、酒精性肝硬化、肝炎后肝硬化和肝细胞癌之间的比较分析显示，肝炎后肝硬化的再生活性最高。
随着当前计算机技术的进步，可以从组织切片进行快速、自动测量，并且可为病理实验室提供各种实际应用，例如用于比较不同类型肝病（包括肝细胞癌分类）的核形态计量学。
