Oguni M, Otani H, Shinohara H, Kato K, Setogawa T
Department of Ophthalmology, Shimane Medical University, Izumo, Japan.
Ophthalmic Res. 1996;28(5):270-4. doi: 10.1159/000267914.
The localization of 28-kD heat shock protein (HSP28) in the lenses of human embryos (Carnegie stages 13-21) was studied immunohistochemically using antibodies for HSP28. At stage 13, HSP28 was observed in the lens placode. At stage 15, when the lens vesicle is formed by the invagination of the lens placode, HSP28 was observed in the posterior portion of the lens vesicle. From stages 16 to 18, when the posterior portion of the lens epithelium elongates toward the anterior lens epithelium and forms the primary lens fiber, HSP28 was observed in the apical and basal sides of the primary lens fiber. At stage 19, when the primary lens fiber attaches to the anterior lens epithelium, immunoreactivity to HSP28 appeared in the anterior lens epithelium. At stage 21, the anterior lens epithelium and the equator portion of the lens, where the secondary lens fiber is formed, were intensely immunoreactive to HSP28. Although alpha B-crystallin is thought to be one of the small HSPs, the distribution of HSP28 in the lenses of human embryos was different from that of alpha B-crystallin which we previously reported.
使用针对28-kD热休克蛋白（HSP28）的抗体，通过免疫组织化学方法研究了人胚胎（卡内基阶段13 - 21）晶状体中HSP28的定位。在第13阶段，在晶状体原基中观察到HSP28。在第15阶段，当晶状体泡由晶状体原基内陷形成时，在晶状体泡的后部观察到HSP28。从第16阶段到第18阶段，当晶状体上皮的后部向前部晶状体上皮延伸并形成初级晶状体纤维时，在初级晶状体纤维的顶端和基部观察到HSP28。在第19阶段，当初级晶状体纤维附着于前部晶状体上皮时，前部晶状体上皮出现对HSP28的免疫反应性。在第21阶段，前部晶状体上皮和形成次级晶状体纤维的晶状体赤道部分对HSP28有强烈的免疫反应性。尽管αB-晶状体蛋白被认为是小热休克蛋白之一，但HSP28在人胚胎晶状体中的分布与我们之前报道的αB-晶状体蛋白的分布不同。
