Casey T M, Chen H, Plaut K, Chiu J F
Department of Animal and Food Sciences, University of Vermont Burlington 05405, USA.
Cell Biol Int. 1996 Nov;20(11):763-7. doi: 10.1006/cbir.1996.0098.
Apoptosis was measured in mammary glands during whole organ culture, to determine whether regression resulting from hormone withdrawal results in epithelial cell death as in vivo involution. Glands were evaluated for morphology and DNA degradation prior to whole organ culture, after lobulo-alveolar development and 2, 4, or 6 days after hormone withdrawal. The data indicated that mammary regression during whole organ culture mimics in vivo involution and results in part from apoptosis of epithelial tissue.
在全器官培养过程中对乳腺中的细胞凋亡进行了检测，以确定激素撤除导致的退化是否会像在体内退化过程中那样引起上皮细胞死亡。在全器官培养前、小叶-腺泡发育后以及激素撤除后2天、4天或6天，对腺体的形态和DNA降解情况进行了评估。数据表明，全器官培养过程中的乳腺退化模拟了体内退化，部分是由上皮组织的细胞凋亡导致的。
