Lavania U C
Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
Indian J Exp Biol. 1996 Aug;34(8):773-5.
Duration of mitotic cell cycle and its component phases in root-tip meristem of P. somniferum was determined following autoradiographic detection of mitotic rythms during the traverse of cell cycle. The mitotic cycle time thus estimated was found to be 12.6h of which the distribution of component phases was G1 = 1.75 h, S = 6.3 h, G2 = 3.75 h and division phase (M) = 0.8 h. Dry seeds were metabolically activated by soaking in water to show that the first batch amongst the asynchronous population of cells in the seeds enters into S phase after 18 h of such soaking at 16 degrees C. Mitotic synchrony to the tune of 80% could be realised when such presoaked seeds were administered 5 mMole hydroxyurea for 20 h at 16 degrees C.
通过对细胞周期进程中丝分裂节律进行放射自显影检测，确定了罂粟根尖分生组织有丝分裂细胞周期及其各组成阶段的持续时间。由此估算出的有丝分裂周期时间为12.6小时，其中各组成阶段的分布为：G1期 = 1.75小时，S期 = 6.3小时，G2期 = 3.75小时，分裂期（M期） = 0.8小时。将干燥种子浸泡在水中使其代谢激活，结果表明，种子中不同步细胞群体中的第一批细胞在16℃下浸泡18小时后进入S期。当将这种预浸泡的种子在16℃下给予5毫摩尔羟基脲处理20小时时，可实现高达80%的有丝分裂同步性。
