槲寄生凝集素I A链的完整氨基酸序列。

Complete amino acid sequence of the A chain of mistletoe lectin I.

作者信息

Huguet Soler M, Stoeva S, Schwamborn C, Wilhelm S, Stiefel T, Voelter W

机构信息

Abteilung für Physikalische Biochemie des Physiologisch-chemischen Instituts der Universität Tübingen, Germany.

出版信息

FEBS Lett. 1996 Dec 9;399(1-2):153-7. doi: 10.1016/s0014-5793(96)01309-9.

DOI:10.1016/s0014-5793(96)01309-9

PMID:8980141

Abstract

The complete amino acid sequence of the A chain of mistletoe lectin I was determined via Edman degradation sequencing of the N-terminus and tryptic and endoproteinase Asp-N overlapping fragments, amino acid analysis and MALDI-MS. The data obtained show a great homology with the chains of ribosome-inactivating proteins such as ricin and abrin with 111 (abrin-a) and 103 (ricin-D) amino acid residues conserved, respectively. The knowledge of the primary structure of MLA will have a fundamental impact on elucidating the biological function of medically applied mistletoe lectins on a molecular basis.

摘要

通过对N端进行埃德曼降解测序以及胰蛋白酶和天冬氨酸蛋白酶Asp-N重叠片段分析、氨基酸分析和基质辅助激光解吸电离质谱法，确定了槲寄生凝集素I A链的完整氨基酸序列。所得数据表明，它与核糖体失活蛋白如蓖麻毒素和相思子毒素的链具有高度同源性，分别有111个（相思子毒素-a）和103个（蓖麻毒素-D）保守氨基酸残基。槲寄生凝集素A链一级结构的知识将对从分子层面阐明医学应用的槲寄生凝集素的生物学功能产生根本性影响。