Kondo S, Shinomura Y, Kanayama S, Higashimoto Y, Miyagawa J I, Minami T, Kiyohara T, Zushi S, Kitamura S, Isozaki K, Matsuzawa Y
Second Department of Internal Medicine, Osaka University Medical School, Yamadaoka, Japan.
Int J Cancer. 1996 Dec 11;68(6):727-30. doi: 10.1002/(SICI)1097-0215(19961211)68:6<727::AID-IJC6>3.0.CO;2-5.
The present study was designed to clarify whether bcl-xL is involved in the development of carcinoma in the stomach. Levels of bcl-xL and bcl-2 mRNA were determined by a reverse-transcription/polymerase-chain reaction in endoscopic gastric biopsy specimens from 10 control subjects, 11 patients with adenomas and 14 patients with carcinomas. In 6 of 11 adenomas, 5 of 8 early carcinomas and 3 of 6 advanced carcinomas, the bcl-xL gene was over-expressed. In carcinomas, over-expression of the bcl-xL gene was observed in 6 of 9 intestinal-type carcinomas and 2 of 5 diffuse-type carcinomas. No correlation was observed between bcl-xL and bcl-2 gene expression. In cases in which the bcl-xL gene was over-expressed, an apparent increase in the protein level of Bcl-xL was observed by immunoblot analysis and intense Bcl-x immunoreactivity was detected immunohistochemically within the tumor cells. In conclusion, we showed that bcl-xL is over-expressed in gastric carcinomas at both the RNA and protein levels, suggesting that over-expression of bcl-xL may play a role in gastric carcinogenesis.
本研究旨在阐明bcl-xL是否参与胃癌的发生发展。通过逆转录/聚合酶链反应测定了10名对照受试者、11名腺瘤患者和14名癌患者的内镜胃活检标本中bcl-xL和bcl-2 mRNA的水平。在11例腺瘤中的6例、8例早期癌中的5例和6例进展期癌中的3例中，bcl-xL基因过度表达。在癌组织中，9例肠型癌中的6例和5例弥漫型癌中的2例观察到bcl-xL基因过度表达。未观察到bcl-xL与bcl-2基因表达之间的相关性。在bcl-xL基因过度表达的病例中，通过免疫印迹分析观察到Bcl-xL蛋白水平明显升高，并且在肿瘤细胞内通过免疫组织化学检测到强烈的Bcl-x免疫反应性。总之，我们表明bcl-xL在胃癌的RNA和蛋白质水平均过度表达，提示bcl-xL的过度表达可能在胃癌发生中起作用。
