Elenitoba-Johnson K S, Medeiros L J, Khorsand J, King T C
Department of Pathology, Roger Williams Medical Center, Rhode Island Hospital, USA.
Am J Clin Pathol. 1996 Dec;106(6):728-38. doi: 10.1093/ajcp/106.6.728.
Immunohistochemically detectable p53 protein expression is common in the Reed-Sternberg and Hodgkin's (RS-H) cells of Hodgkin's disease, but p53 gene mutations have only rarely been identified. The authors found p53 expression in RS-H cells in 16 of 30 cases of Hodgkin's disease (53%), with the percentage of RS-H positive cells ranging from 4% to 85%. In 12 of 30 cases (40%), at least 10% of the RS-H cells were positive for p53. p53 gene mutations were detected in only two cases (7%) using a single-stranded conformational polymorphism assay with a detection sensitivity of between 1% and 5%. The cellular protein, mdm-2, which can stabilize and functionally inactivate wild-type p53 protein, was expressed in RS-H cells in most of these cases (86%). However, neither case with a p53 gene mutation expressed mdm-2 (P < .005). The two cases with p53 gene mutations had a higher mean proliferative index than cases without detectable mutations (90% versus 72%; P < .02). p53 expression in RS-H cells may be related to concurrent mdm-2 protein expression and a p53-positive, mdm-2-negative immunophenotype may be predictive of gene mutations in RS-H cells.
免疫组化可检测到的p53蛋白表达在霍奇金病的里德-斯腾伯格细胞（RS-H细胞）中很常见，但p53基因突变却很少被发现。作者在30例霍奇金病患者中的16例（53%）的RS-H细胞中发现了p53表达，RS-H阳性细胞的百分比在4%至85%之间。在30例中的12例（40%）中，至少10%的RS-H细胞p53呈阳性。使用检测灵敏度在1%至5%之间的单链构象多态性分析，仅在2例（7%）中检测到p53基因突变。细胞蛋白mdm-2可稳定野生型p53蛋白并使其功能失活，在大多数此类病例（86%）的RS-H细胞中均有表达。然而，两例p53基因突变的病例均未表达mdm-2（P<0.005）。两例p53基因突变的病例的平均增殖指数高于未检测到突变的病例（90%对72%；P<0.02）。RS-H细胞中的p53表达可能与mdm-2蛋白的同时表达有关，p53阳性、mdm-2阴性免疫表型可能预示着RS-H细胞中的基因突变。
