Koziel M G, Carozzi N B, Desai N
Ciba Agricultural Biotechnology Unit, Research Triangle Park, NC 27709, USA.
Plant Mol Biol. 1996 Oct;32(1-2):393-405. doi: 10.1007/BF00039392.
Introducing a foreign gene into a new plant host does not always result in a high level of expression of the incoming gene. Numerous promoters have been used to express foreign genes in different plant tissues, but there are sometime various features of the new gene which are deleterious to expression in the new host. There are a number of post-transcriptional steps in the expression of a gene and sometimes sequences present in a particular coding region can resemble the signals which initiate these processing steps. When aberrantly carried out, these steps diminish the level of expression. By removing such fortuitous signals, one can dramatically increase expression of a transgene in plants. Ensuring proper protein folding and/or targeting the protein product to a particular cellular compartment can also be used to increase the level of protein obtained. The various methods used to optimize expression of a foreign gene in plants by concentrating on post-transcriptional events are discussed.
将外源基因导入新的植物宿主并不总是能使导入基因高水平表达。众多启动子已被用于在不同植物组织中表达外源基因，但新基因有时存在各种不利于在新宿主中表达的特性。基因表达过程中有许多转录后步骤，有时特定编码区域中存在的序列可能类似于启动这些加工步骤的信号。当这些步骤异常进行时，会降低表达水平。通过去除此类偶然信号，能够显著提高转基因在植物中的表达。确保蛋白质正确折叠和/或将蛋白质产物靶向特定细胞区室也可用于提高所获得蛋白质的水平。本文讨论了通过关注转录后事件来优化外源基因在植物中表达的各种方法。
