Choi S Y
Department of Microbiology, HanNam University, Taejon, Republic of Korea.
Plant Mol Biol. 1996 Dec;32(5):995-7. doi: 10.1007/BF00020496.
The 22 kDa auxin-binding proteins in higher plants have received considerable attention as candidates for an auxin receptor. A cDNA clone Ca-ERabp1 of hot pepper (Capsicum annum) was isolated using the oligonucleotides as PCR primers. The cDNA codes for a polypeptide related to the major 22 kDa auxin-binding protein from maize and Arabidopsis ERabp1. The deduced amino acid sequence contains an endoplasmic reticulum retention signal, the KDEL sequence located at the C-terminal end, and has two possible auxin-binding sites, HRHSCE and YDDWSVPHTA conserved sequences. Northern hybridization analysis revealed that the Ca-ERabp1 gene is differentially expressed in total RNA isolated from different organs of a pepper plant, showing the highest level of expression in fruits but barely detectable in leaves and roots.
高等植物中22 kDa的生长素结合蛋白作为生长素受体的候选者受到了广泛关注。以寡核苷酸为PCR引物，分离出了辣椒（Capsicum annum）的一个cDNA克隆Ca-ERabp1。该cDNA编码一种与玉米和拟南芥ERabp1中主要的22 kDa生长素结合蛋白相关的多肽。推导的氨基酸序列包含一个内质网滞留信号，即位于C末端的KDEL序列，并且有两个可能的生长素结合位点，即HRHSCE和YDDWSVPHTA保守序列。Northern杂交分析表明，Ca-ERabp1基因在从辣椒植株不同器官分离的总RNA中差异表达，在果实中的表达水平最高，而在叶和根中几乎检测不到。
