Coker R K, Laurent G J, Shahzeidi S, Hernández-Rodríguez N A, Pantelidis P, du Bois R M, Jeffery P K, McAnulty R J
Centre for Cardiopulmonary Biochemistry & Respiratory Medicine, University College London Medical School, University of London, UK.
Eur Respir J. 1996 Dec;9(12):2501-7. doi: 10.1183/09031936.96.09122501.
A role for transforming growth factor-beta 1, (TGF-beta 1) has been proposed in lung development and in the pathogenesis of pulmonary disease. However, previous studies have not delineated the cells expressing TGF-beta 1 in normal adult lung, nor compared its gene expression with that of other TGF-beta isoforms. We used digoxigenin-labelled riboprobes to localize TGF-beta 1 and TGF-beta 3 gene expression in normal adult human and mouse lung. This procedure was technically simple, providing excellent resolution. TGF-beta 1 and TGF-beta 3 messenger ribonucleic acid (mRNA) transcripts were detected in a wide variety of cells. In human lung, mRNA for both isoforms was localized to bronchiolar epithelium and alveolar macrophages. TGF-beta 1, but not TGF-beta 3 mRNA was detected in mesenchymal and endothelial cells. In murine tissue, TGF-beta 1, mRNA was localized to bronchiolar epithelium, Clara cells, mesenchymal cells, pulmonary endothelium and alveolar cells, including macrophages. TGF-beta 3 mRNA was similarly distributed but not detected in endothelium. In summary, using a nonisotopic technique in lung tissue, we have detailed the cells expressing the transforming growth factor-beta 1 and beta 3 genes in human and murine lung. There was widespread expression of these cytokines in normal lung consistent with autocrine or paracrine roles in regulating cellular turnover, immune defence and matrix protein metabolism.
转化生长因子-β1（TGF-β1）在肺发育及肺部疾病发病机制中所起的作用已被提出。然而，以往研究尚未明确正常成年肺中表达TGF-β1的细胞，也未将其基因表达与其他TGF-β亚型进行比较。我们使用地高辛标记的核糖探针来定位正常成年人类和小鼠肺中TGF-β1和TGF-β3的基因表达。该方法技术简单，分辨率极佳。在多种细胞中检测到了TGF-β1和TGF-β3信使核糖核酸（mRNA）转录本。在人类肺中，两种亚型的mRNA均定位于细支气管上皮和肺泡巨噬细胞。在间充质细胞和内皮细胞中检测到了TGF-β1 mRNA，但未检测到TGF-β3 mRNA。在鼠组织中，TGF-β1 mRNA定位于细支气管上皮、克拉拉细胞、间充质细胞、肺内皮细胞和肺泡细胞，包括巨噬细胞。TGF-β3 mRNA分布类似，但在内皮细胞中未检测到。总之，通过在肺组织中使用非同位素技术，我们详细阐述了人类和鼠肺中表达转化生长因子-β1和β3基因的细胞。这些细胞因子在正常肺中广泛表达，这与它们在调节细胞更新、免疫防御和基质蛋白代谢中的自分泌或旁分泌作用相一致。
