Morgenthaler N G, Scherbaum W A, Banga J P
Department of Internal Medicine III, University of Leipzig, Germany.
Exp Clin Endocrinol Diabetes. 1996;104 Suppl 4:56-9. doi: 10.1055/s-0029-1211703.
Immunoprecipitation is a reliable method for the detection of many antigens but so far this has not been applied to the TSH receptor. We demonstrate here a novel assay for the detection of antibodies to the TSH receptor using in vitro synthesized protein. The TSH receptor was translated in vitro as a stable protein of 87 kD (full length) and 50 kD (extracellular), respectively. The 50 kD protein reacted by immunoprecipitation analysis with murine and human monoclonal antibodies, polyclonal rabbit sera and with a proportion of unfractionated Graves' disease sera. A limited form of epitope mapping was achieved with the murine monoclonal antibodies. This assay may serve as a new method for the detection of autoantibodies to the TSH receptor from patients' sera.
免疫沉淀法是检测多种抗原的可靠方法，但迄今为止尚未应用于促甲状腺激素（TSH）受体。我们在此展示一种利用体外合成蛋白检测TSH受体抗体的新方法。TSH受体在体外分别被翻译为87kD（全长）和50kD（细胞外）的稳定蛋白。通过免疫沉淀分析，50kD蛋白与鼠源和人源单克隆抗体、兔多克隆血清以及一部分未经分离的格雷夫斯病血清发生反应。利用鼠源单克隆抗体实现了有限形式的表位作图。该检测方法可作为从患者血清中检测TSH受体自身抗体的新方法。
