Settles D L, Kusakabe M, Steindler D A, Fillmore H, Erickson H P
Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.
J Neurosci Res. 1997 Jan 1;47(1):109-17.
A recent study by Mitrovic and Schachner (J Neurosci Res 42:710-717, 1995) reported the detection of a small amount of truncated tensacin-C (TN-C) in the nervous system of the TN-C knockout mice created by Saga et al. (Genes Dev 6:1821-1831, 1992). The authors suggested that the truncated protein might be responsible for the failure to detect any phenotypic abnormalities in the knockout mice. We have reexamined the knockout mice in our laboratories by Western blot and immunocytochemistry, and have not detected any full-length or truncated TN-C protein. In addition, we note that the construction of the knockout gene deleted the signal sequence, so if any residual truncated protein were produced it would be trapped in the cytoplasm, and therefore inaccessible to extracellular ligands or receptors. We therefore conclude that the TN-C knockout created by Saga et al. is a valid TN-C null.
米特罗维奇和沙克纳最近的一项研究（《神经科学研究杂志》42:710 - 717, 1995）报告称，在由佐贺等人构建的（《基因与发育》6:1821 - 1831, 1992）腱生蛋白-C（TN-C）基因敲除小鼠的神经系统中检测到少量截短的TN-C。作者认为，这种截短蛋白可能是在基因敲除小鼠中未检测到任何表型异常的原因。我们在实验室通过蛋白质免疫印迹法和免疫细胞化学法对这些基因敲除小鼠进行了重新检测，未检测到任何全长或截短的TN-C蛋白。此外，我们注意到基因敲除构建体删除了信号序列，所以如果产生任何残留的截短蛋白，它将被困在细胞质中，因此细胞外配体或受体无法接触到。因此，我们得出结论，佐贺等人构建的TN-C基因敲除小鼠是有效的TN-C基因无效模型。
