Munn E A
Tissue Cell. 1977;9(1):23-34. doi: 10.1016/0040-8166(77)90046-5.
The structure of the intestinal cells of the parasitic nematode Haemonchus contortus is described. The cells have numerous microvilli about 0-09 micron in diameter; most being 5-5-7-5 micron in length. The microvillar (plasma) membrane is coated with a layer of amorphous material (glycocalyx) about 60 A thick which is electron dense in sectioned preparations. Associated with the surface of this material, and filing the spaces between the microvilli, are filaments in the form of helices about 400 A in diameter and of variable pitch. The helices appear to be flexible but they are aligned approximately with the long axes of the microvilli. There are up to ten helices per microvillus; they extend beyond the tips of the microvilli and are up to 10 micron long. The material has been obtained nearly pure in small amounts. It is primarily protein and it is proposed that it should be called contortin. The monomeric form (of molecular weight about 60,000) has been identified with a Y-shaped structure with arms about 45 A long and 25 A wide seen in negatively stained preparations. The helical filament appears to be formed by lateral polymerization of patirs of these units.
本文描述了寄生线虫捻转血矛线虫肠道细胞的结构。这些细胞有许多直径约0.09微米的微绒毛；大多数微绒毛长度为5.5 - 7.5微米。微绒毛（质膜）表面覆盖着一层约60埃厚的无定形物质（糖萼），在切片标本中该物质电子密度较高。与这种物质表面相关并填充微绒毛之间间隙的是直径约400埃、螺距可变的螺旋状细丝。这些螺旋看起来是可弯曲的，但它们大致与微绒毛的长轴对齐。每个微绒毛上最多有十条螺旋；它们延伸到微绒毛尖端之外，长度可达10微米。已少量获得了近乎纯净的该物质。其主要成分是蛋白质，建议将其命名为捻转蛋白。在负染标本中已鉴定出单体形式（分子量约60,000）呈Y形结构，臂长约45埃、宽约25埃。螺旋细丝似乎是由这些单元的成对侧向聚合形成的。
