Kohsaka H, Carson D A, Rassenti L Z, Ollier W E, Chen P P, Kipps T J, Miyasaka N
Division of Immunological Diseases, Medical Research Institute, Tokyo Medical and Dental University, Japan.
J Clin Invest. 1996 Dec 15;98(12):2794-800. doi: 10.1172/JCI119106.
The factors controlling immunoglobulin (Ig) gene repertoire formation are poorly understood. Studies on monozygotic twins have helped discern the contributions of genetic versus environmental factors on expressed traits. In the present experiments, we applied a novel anchored PCR-ELISA system to compare the heavy chain V gene (V(H)) subgroup repertoires of mu and gamma expressing B lymphocytes from ten pairs of adult monozygotic twins, including eight pairs who are concordant or discordant for rheumatoid arthritis. The results disclosed that the relative expression of each Ig V(H) gene subgroup is not precisely proportional to its relative genomic size. The monozygotic twins had more similar IgM V(H) gene repertoires than did unrelated subjects. Moreover, monozygotic twins who are discordant for RA also use highly similar IgM V(H) gene-subgroup repertoires. Finally, the V(H) gene repertoire remained stable over time. Collectively, these data reveal that genetic factors predominantly control V(H) gene repertoire formation.
目前对控制免疫球蛋白（Ig）基因库形成的因素了解甚少。对同卵双胞胎的研究有助于区分遗传因素与环境因素对表达性状的影响。在本实验中，我们应用了一种新型的锚定PCR-ELISA系统，比较了来自十对成年同卵双胞胎（包括八对患类风湿性关节炎一致或不一致的双胞胎）的表达μ链和γ链的B淋巴细胞的重链V基因（V(H)）亚群库。结果显示，每个Ig V(H)基因亚群的相对表达与其相对基因组大小并不精确成正比。同卵双胞胎的IgM V(H)基因库比无关个体更为相似。此外，患类风湿性关节炎不一致的同卵双胞胎也使用高度相似的IgM V(H)基因亚群库。最后，V(H)基因库随时间保持稳定。总体而言，这些数据表明遗传因素主要控制V(H)基因库的形成。
