酵母人工染色体（YAC）的HAPPY图谱揭示了人类免疫球蛋白VH基因座中的替代单倍型。
HAPPY mapping of a YAC reveals alternative haplotypes in the human immunoglobulin VH locus.
作者信息
Walter G, Tomlinson I M, Cook G P, Winter G, Rabbitts T H, Dear P H
机构信息
MRC Centre for Protein Engineering, Cambridge, UK.
出版信息
Nucleic Acids Res. 1993 Sep 25;21(19):4524-9. doi: 10.1093/nar/21.19.4524.
We have identified and sequenced 14 human immunoglobulin VH segments cloned in a yeast artificial chromosome, and have used a rapid PCR-based technique (HAPPY mapping, 12) to derive the order and approximate distances between them. The sequences mapped comprise thirteen germline VH segments and one rearranged VH3 gene. Comparison of our map with other data suggests the existence of at least two distinct haplotypes, differing in the presence or absence of the consecutive genes DP-78, DP-46 and DP-64, and in the duplication of segments DP-49 and DP-65. Screening of ten individuals confirms the existence of both haplotypes, and indicates that both are common amongst the population.
我们已经鉴定并测序了克隆于酵母人工染色体中的14个人类免疫球蛋白VH片段，并使用了一种基于聚合酶链反应的快速技术（HAPPY作图法，参考文献12）来确定它们之间的顺序和大致距离。所绘制图谱的序列包括13个种系VH片段和1个重排的VH3基因。将我们的图谱与其他数据进行比较表明，至少存在两种不同的单倍型，它们在连续基因DP - 78、DP - 46和DP - 64的有无以及片段DP - 49和DP - 65的重复情况上存在差异。对10个人的筛查证实了这两种单倍型的存在，并表明它们在人群中都很常见。
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