sspF基因失活或过表达对枯草芽孢杆菌芽孢特性的影响。
Effects of inactivation or overexpression of the sspF gene on properties of Bacillus subtilis spores.
作者信息
Loshon C A, Kraus P, Setlow B, Setlow P
机构信息
Department of Natural Sciences, University of Maryland Eastern Shore, Princess Anne 21853, USA.
出版信息
J Bacteriol. 1997 Jan;179(1):272-5. doi: 10.1128/jb.179.1.272-275.1997.
Abstract
Inactivation of the Bacillus subtilis sspF gene had no effect on sporulation, spore resistance, or germination in a wild-type strain or one lacking DNA protective alpha/beta-type small, acid-soluble proteins (SASP). Overexpression of SspF in wild-type spores or in spores lacking major alpha/beta-type SASP (alpha- beta- spores) had no effect on sporulation but slowed spore outgrowth and restored a small amount of UV and heat resistance to alpha- beta- spores. In vitro analyses showed that SspF is a DNA binding protein and is cleaved by the SASP-specific protease (GPR) at a site similar to that cleaved in alpha/beta-type SASP. SspF was also degraded during spore germination and outgrowth, and this degradation was initiated by GPR.
摘要
枯草芽孢杆菌sspF基因的失活对野生型菌株或缺乏DNA保护型α/β型小酸溶性蛋白（SASP）的菌株的孢子形成、孢子抗性或萌发没有影响。在野生型孢子或缺乏主要α/β型SASP的孢子（α-β-孢子）中过表达SspF对孢子形成没有影响，但减缓了孢子的萌发，并恢复了α-β-孢子的少量紫外线和热抗性。体外分析表明，SspF是一种DNA结合蛋白，并且在与α/β型SASP中被切割的位点相似的位置被SASP特异性蛋白酶（GPR）切割。SspF在孢子萌发和生长过程中也会降解，这种降解由GPR启动。
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