Mason J M, Setlow P
J Bacteriol. 1986 Jul;167(1):174-8. doi: 10.1128/jb.167.1.174-178.1986.
Bacillus subtilis strains containing deletions in the genes coding for one or two of the major small, acid-soluble spore proteins (SASP; termed SASP-alpha and SASP-beta) were constructed. These mutants sporulated normally, but the spores lacked either SASP-alpha, SASP-beta, or both proteins. The level of minor SASP did not increase in these mutants, but the level of SASP-alpha increased about twofold in the SASP-beta- mutant, and the level of SASP-beta increased about twofold in the SASP-alpha- mutant. The growth rates of the deletion strains were identical to that of the wild-type strain in rich or poor growth media, as was the initiation of spore germination. However, outgrowth of spores of the SASP-alpha(-)-beta- strain was significantly slower than that of wild-type spores in all media tested. The heat resistance of SASP-beta- spores was identical to that of wild-type spores but slightly greater than that of SASP-alpha- and SASP-alpha(-)-beta- spores. However, the SASP-alpha- and SASP-alpha(-)-beta- spores were much more heat resistant than vegetative cells. The UV light resistances of SASP-beta- and wild-type spores were also identical. However, SASP-alpha(-)-beta- spores were slightly more sensitive to UV light than were log-phase cells of the wild-type or SASP-alpha(-)-beta- strain (the latter have identical UV light resistances); SASP-alpha- spores were slightly more UV light resistant than SASP-alpha(-)-beta- spores. These data strongly implicate SASP, in particular SASP-alpha, in the UV light resistance of B. subtilis spores.
构建了编码一种或两种主要的小酸性可溶性芽孢蛋白（SASP；称为SASP-α和SASP-β）的基因缺失的枯草芽孢杆菌菌株。这些突变体正常形成芽孢，但芽孢缺乏SASP-α、SASP-β或这两种蛋白。在这些突变体中，次要SASP的水平没有增加，但在SASP-β缺失突变体中，SASP-α的水平增加了约两倍，在SASP-α缺失突变体中，SASP-β的水平增加了约两倍。在丰富或贫瘠的生长培养基中，缺失菌株的生长速率与野生型菌株相同，芽孢萌发的起始情况也相同。然而，在所有测试培养基中，SASP-α（-）-β（-）菌株芽孢的出芽明显慢于野生型芽孢。SASP-β缺失芽孢的耐热性与野生型芽孢相同，但略高于SASP-α缺失芽孢和SASP-α（-）-β（-）芽孢。然而，SASP-α缺失芽孢和SASP-α（-）-β（-）芽孢比营养细胞耐热得多。SASP-β缺失芽孢和野生型芽孢的抗紫外线能力也相同。然而，SASP-α（-）-β（-）芽孢对紫外线的敏感性略高于野生型或SASP-α（-）-β（-）菌株对数期细胞（后者具有相同的抗紫外线能力）；SASP-α缺失芽孢比SASP-α（-）-β（-）芽孢略耐紫外线。这些数据有力地表明SASP，特别是SASP-α，与枯草芽孢杆菌芽孢的抗紫外线能力有关。
