一段53个碱基对的反向重复序列对巨大芽孢杆菌中相邻且方向相反的细胞色素P450（BM - 1）基因及其调控基因bm1P1的表达具有负调控作用。
A 53-base-pair inverted repeat negatively regulates expression of the adjacent and divergently oriented cytochrome P450(BM-1) gene and its regulatory gene, bm1P1, in Bacillus megaterium.
作者信息
Shaw G C, Sung C C, Liu C H, Kao H S
机构信息
Institute of Biochemistry, School of Life Science, National Yang-Ming University, Taipei, Taiwan, Republic of China.
出版信息
J Bacteriol. 1997 Jan;179(1):280-3. doi: 10.1128/jb.179.1.280-283.1997.
Abstract
To study the role of the cis-acting element(s) in controlling the expression of the cytochrome P450(BM-1) gene and its upstream regulatory gene, bm1P1, in Bacillus megaterium, various deletion derivatives were constructed. A 53-bp inverted repeat located midway between the P450(BM-1) gene and bm1P1 gene was found in vivo to negatively regulate the expression of both genes, the regulation of which may occur at the transcriptional level. The promoter of the P450(BM-1), gene was also identified and found to be similar to those recognized by the sigmaA RNA polymerase of Bacillus subtilis. Possible mechanisms by which the 53-bp inverted repeat regulates the gene expression are discussed.
摘要
为了研究顺式作用元件在巨大芽孢杆菌中控制细胞色素P450（BM-1）基因及其上游调控基因bm1P1表达中的作用，构建了各种缺失衍生物。在体内发现位于P450（BM-1）基因和bm1P1基因中间位置的一个53bp反向重复序列对这两个基因的表达均有负调控作用，这种调控可能发生在转录水平。还鉴定了P450（BM-1）基因的启动子，发现它与枯草芽孢杆菌的σA RNA聚合酶识别的启动子相似。讨论了53bp反向重复序列调控基因表达的可能机制。
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