Marcus N, Shaffer D, Farrar P, Green M
Department of Molecular Microbiology and Immunology, St. Louis University School of Medicine, MO 63104, USA.
Biochim Biophys Acta. 1996 Dec 11;1309(3):253-60. doi: 10.1016/s0167-4781(96)00133-9.
The distribution of PDI, ERp61 and ERp72, members of the protein disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins, was determined in various murine tissues. Relative amounts of mRNA were measured using a quantitative reverse transcription-polymerase chain reaction (RT-PCR) method. Protein levels were determined from Western blots. In most tissues, protein levels paralleled the amount of mRNA for each PDI family member. The tissue distribution of the PDI and ERp72 mRNAs was similar, although ERp72 was not as abundant as PDI in the tissues. The tissue distribution of the ERp61 mRNA was significantly different from the two other family members. To help define potential hormonal or maturational differences in the regulation of expression of PDI family members, mRNA was measured in the frontal cortex, liver, pituitary gland and uterus at timed intervals during postnatal maturation. Except in the pituitary gland, the mRNA levels at 10 days and 22 days after birth were essentially identical to those in the adult. The ERp61 and ERp72 mRNAs were present at 2- to 3-fold higher levels in the pituitary glands of the 10- and 22-day-old mice, than in the adult mice. In addition, the pituitary gland PDI mRNA was 2- to 3-fold higher in 10-day-old mice than in adults. In general, levels of PDI family members were higher in secretory tissues than in other tissues in both immature and adult mice.
内质网（ER）蛋白的蛋白质二硫键异构酶（PDI）家族成员PDI、ERp61和ERp72在多种小鼠组织中的分布已被确定。使用定量逆转录-聚合酶链反应（RT-PCR）方法测量mRNA的相对含量。通过蛋白质印迹法测定蛋白质水平。在大多数组织中，每个PDI家族成员的蛋白质水平与mRNA的量平行。PDI和ERp72 mRNA的组织分布相似，尽管ERp72在组织中的含量不如PDI丰富。ERp61 mRNA的组织分布与其他两个家族成员显著不同。为了帮助确定PDI家族成员表达调控中潜在的激素或成熟差异，在出生后成熟过程中的不同时间间隔，测量额叶皮质、肝脏、垂体和子宫中的mRNA。除垂体外，出生后10天和22天的mRNA水平与成年小鼠基本相同。10日龄和22日龄小鼠垂体中的ERp61和ERp72 mRNA水平比成年小鼠高2至3倍。此外，10日龄小鼠垂体中的PDI mRNA比成年小鼠高2至3倍。一般来说，在未成熟和成年小鼠中，分泌组织中PDI家族成员的水平均高于其他组织。
