Venkatesh N, Murthy G S
Primate Research Laboratory, Indian Institute of Science, Bangalore, India.
J Immunol Methods. 1996 Dec 15;199(2):167-74. doi: 10.1016/s0022-1759(96)00179-2.
A single step solid phase radioimmunoassay (SS-SPRIA) has been developed for human chorionic gonadotropin (hCG) using monoclonal antibodies (MAb) from culture media adsorbed immunochemically on plastic tubes. The assays have been found to be very simple in terms of operation and do not demand purification of MAbs. Several MAbs which do not show any displacement in liquid phase RIA and ELISA provide a satisfactory SS-SPRIA. Our investigations revealed that the assumption regarding the stability of the primary Mab-Ag complex during incubation and washing steps in ELISAs is not strictly valid for dissociable MAbs. A comparison of different assay systems suggests that the single step SPRIA offers additional advantages over conventionally used multistep ELISA procedures and provides a quantitative probe for the analysis of epitope-paratope interactions.
已开发出一种用于人绒毛膜促性腺激素（hCG）的单步固相放射免疫分析（SS-SPRIA）方法，该方法使用通过免疫化学吸附在塑料管上的来自培养基的单克隆抗体（MAb）。已发现该分析方法在操作方面非常简单，并且不需要纯化单克隆抗体。在液相放射免疫分析和酶联免疫吸附测定中未显示任何置换作用的几种单克隆抗体可提供令人满意的单步固相放射免疫分析。我们的研究表明，关于酶联免疫吸附测定中孵育和洗涤步骤期间一级单克隆抗体-抗原复合物稳定性的假设对于可解离的单克隆抗体并不严格成立。不同分析系统的比较表明，单步固相放射免疫分析比传统使用的多步酶联免疫吸附测定方法具有更多优势，并为表位-互补位相互作用的分析提供了一种定量探针。
