Koyama Y, Ichikawa T, Nakano E
Research and Development Division, Kikkoman Corporation, Chiba.
J Biochem. 1996 Nov;120(5):969-73. doi: 10.1093/oxfordjournals.jbchem.a021514.
A urate oxidase (uricase) gene was cloned from Candida utilis with an oligonucleotide probe based on the amino acid sequence of cyanogen bromide-cleaved uricase. The uricase gene contains 909 base pairs and encodes a protein with a predicted mass of 34,193 Da. Candida uricase was similar (49% match in amino acid sequence) to the uricase from Aspergillus flavus. The uricase from Candida utilis has four cysteines and one of them, Cys168, participates in the enzyme activity. This enzyme was expressed to a level of about 20% of total cellular protein in an Escherichia coli cell as a soluble and functional form.
基于溴化氰裂解的尿酸酶氨基酸序列，用寡核苷酸探针从产朊假丝酵母中克隆了尿酸氧化酶（尿酸酶）基因。该尿酸酶基因包含909个碱基对，编码一种预测分子量为34,193 Da的蛋白质。产朊假丝酵母尿酸酶与黄曲霉尿酸酶相似（氨基酸序列匹配度为49%）。产朊假丝酵母尿酸酶有四个半胱氨酸，其中一个半胱氨酸Cys168参与酶活性。该酶在大肠杆菌细胞中以可溶且有功能的形式表达，表达水平约占总细胞蛋白的20%。
