牛对捻转血矛线虫排泄分泌抗原的体液免疫反应。

Bovine humoral immune responses to Haemonchus placei excretory secretory antigens.

作者信息

Schallig H D, Moyo D Z, Hendrikx W M, Eysker M

机构信息

Utrecht University, Department of Parasitology & Tropical Veterinary Medicine, Netherlands.

出版信息

Vet Parasitol. 1996 Oct 25;65(3-4):289-96. doi: 10.1016/s0304-4017(96)00950-8.

DOI:10.1016/s0304-4017(96)00950-8

PMID:8983155

Abstract

The immune response of calves against excretory secretory (ES) products of adult Haemonchus placei was studied. The ES products of adult H. placei comprise at least ten polypeptides with molecular weights ranging from 10 to over 100 kDa. A primary infection of calves with H. placei did not result in a marked elevation of IgG serum antibodies reactive to ES antigens as demonstrated by ELISA. Immunoblotting experiments revealed that sera of primary infected calves recognised a 50 kDa antigen in most cases. An increase in the level of serum IgG antibodies was observed after trickle infections. The secondary immune response coincided with the recognition of a group of three ES antigens of around 50-55 kDa and one protein with a molecular weight of 24 kDa.

摘要

研究了犊牛对成年柏氏血矛线虫排泄分泌（ES）产物的免疫反应。成年柏氏血矛线虫的ES产物包含至少十种分子量范围从10 kDa至超过100 kDa的多肽。如ELISA所示，犊牛初次感染柏氏血矛线虫不会导致对ES抗原反应的IgG血清抗体显著升高。免疫印迹实验表明，初次感染犊牛的血清在大多数情况下识别出一种50 kDa的抗原。在持续感染后观察到血清IgG抗体水平增加。二次免疫反应与识别一组分子量约为50 - 55 kDa的三种ES抗原和一种分子量为24 kDa的蛋白质同时发生。