Hardtke C S, Berleth T
Institut für Genetik und Mikrobiologie, Universität München, Germany.
Genome. 1996 Dec;39(6):1086-92. doi: 10.1139/g96-136.
In the course of the isolation of the MONOPTEROS (MP) gene, required for primary root formation in Arabidopsis thaliana, a yeast artificial chromosome (YAC) contig encompassing approximately 2200 kilobases corresponding to 5.5 cM on the top arm of chromosome 1 was established. Forty-six YAC clones were characterized and 12 new restriction fragment length polymorphism (RFLP) markers are presented. Three new codominant amplified polymorphic sequence (CAPS) markers were generated that enabled high resolution genetic mapping and correlation of physical and genetic distances along the contig. The map contributes to the completion of a physical map of the Arabidopsis genome and should facilitate positional cloning of other genes in the region as well as studies on genome organization. We also present another set of 11 physically linked probes, as well as mapping data for additional RFLP markers within a broader interval of 10.4 cM.
在分离拟南芥主根形成所需的MONOPTEROS（MP）基因的过程中，构建了一个酵母人工染色体（YAC）重叠群，其覆盖了约2200千碱基，相当于第1号染色体长臂上5.5厘摩的区域。对46个YAC克隆进行了特征分析，并展示了12个新的限制性片段长度多态性（RFLP）标记。产生了3个新的共显性扩增多态性序列（CAPS）标记，这些标记能够进行高分辨率遗传作图，并使重叠群上的物理距离与遗传距离相关联。该图谱有助于完成拟南芥基因组的物理图谱，并且应该便于该区域其他基因的定位克隆以及基因组组织的研究。我们还展示了另一组11个物理连锁探针，以及在更宽的10.4厘摩区间内其他RFLP标记的作图数据。
