Chung Y C, Huang C, Tseng C P
Institute of Environmental Engineering, National Chiao Tung University, Hsinchu, Taiwan, Republic of China.
Biotechnol Prog. 1996 Nov-Dec;12(6):773-8. doi: 10.1021/bp960058a.
A heterotrophic Pseudomonas putida CH11 was isolated from livestock farming wastewater and applied for the treatment of H2S-containing gas. Extensive tests including removal characteristics, metabolic products, and removal efficiencies of H2S by P. putida CH11 were examined in batch and continuous systems. The optimum pH required to remove hydrogen sulfide was found in the range of 6-8. The maximum removal rate and the saturation constant were calculated to be Vm = 1.36 g S/day.kg dry bead and Ks = 45.9 ppm, respectively. The main metabolic product of H2S oxidation was determined to be elemental sulfur. When P. putida CH11 was immobilized within Ca alginate, the cells exhibited high H2S removal efficiency, in excess of 96%, at concentration of hydrogen sulfide from 10 to 150 ppm (flow rates of 36 and 72 L/h). These results suggest that P. putida CH11 immobilized within Ca alginate has the potential to be used as a H2S removal agent.
从畜牧养殖废水中分离出一株异养恶臭假单胞菌CH11，并将其应用于含硫化氢气体的处理。在间歇式和连续式系统中，对恶臭假单胞菌CH11去除硫化氢的特性、代谢产物及去除效率等进行了广泛测试。发现去除硫化氢所需的最佳pH值范围为6 - 8。计算得出最大去除率和饱和常数分别为Vm = 1.36 g S/天·kg干珠和Ks = 45.9 ppm。硫化氢氧化的主要代谢产物确定为元素硫。当恶臭假单胞菌CH11固定在海藻酸钙中时，在硫化氢浓度为10至150 ppm（流速为36和72 L/h）时，细胞表现出超过96%的高硫化氢去除效率。这些结果表明，固定在海藻酸钙中的恶臭假单胞菌CH11有潜力用作硫化氢去除剂。
