载脂蛋白E在肾淀粉样变性中的免疫组织化学定位

Immunohistochemical localization of apolipoprotein E in renal amyloidosis.

作者信息

Taniguchi Y, Yorioka N, Yamashita K, Oda H, Nie L F, Ye Yeu X F, Okushin S, Nishida Y, Kushihata S, Yamakido M

机构信息

Second Department of Internal Medicine, Hiroshima University School of Medicine, Japan.

出版信息

Hiroshima J Med Sci. 1996 Mar;45(1):11-4.

PMID:8984100

Abstract

To clarify the role of apolipoprotein E (apo E) in the formation of amyloid deposits, we examined specimens from 11 patients with renal amyloidosis who underwent renal biopsy by an immunohistochemical method using a monoclonal antibody (murine IgG1). Apo E was distributed in the amyloid deposits of all patients in a pattern similar to that obtained with Congo red staining. Strong positive staining for apo E was found on the amyloid deposits in the glomeruli. These results suggest that apo E is a common constituent of amyloid fibrils and that it may be a useful marker for immunohistochemical studies of systemic amyloidosis including renal amyloidosis.

摘要

为阐明载脂蛋白E（apo E）在淀粉样沉积物形成中的作用，我们采用单克隆抗体（鼠IgG1）免疫组化方法，检测了11例接受肾活检的肾淀粉样变性患者的标本。apo E以与刚果红染色相似的模式分布于所有患者的淀粉样沉积物中。在肾小球的淀粉样沉积物上发现apo E呈强阳性染色。这些结果表明，apo E是淀粉样纤维的常见成分，并且它可能是包括肾淀粉样变性在内的系统性淀粉样变性免疫组化研究的有用标志物。