Paterson M, Wilson M R, van Duin M, Aitken R J
MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh, UK.
J Reprod Fertil Suppl. 1996;50:175-82.
Antibodies directed against the zona pellucida can interrupt sperm-egg recognition in vitro. However, the mechanisms by which anti-zona antibodies exert this contraceptive effect in vivo remain uncertain. There is an accumulating body of evidence to suggest that active immunity against zona antigens not only induces infertility via an antibody-mediated interruption of sperm--egg interaction but also disrupts normal ovarian function. We have evaluated the consequence of inducing active immunity against purified recombinant human ZP3 (rec.hZP3) and human ZP3 peptides, using the marmoset monkey, Callithrix jacchus, as an animal model. Although infertility was established in animals that received rec.hZP3, it was associated with ovarian dysfunction characterized by suppression of folliculogenesis and depletion of the primordial follicle pool. Immunization with continuous human ZP3 peptides, identified by epitope mapping studies, did not induce ovarian pathology but the antibody titres were insufficient to suppress fertility significantly and consistently. Clearly, further research is required to identify and segregate epitopes on the zona glycoproteins that are capable of inducing infertility without any side effects.
针对透明带的抗体可在体外阻断精子与卵子的识别。然而，抗透明带抗体在体内发挥这种避孕作用的机制仍不明确。越来越多的证据表明，针对透明带抗原的主动免疫不仅通过抗体介导的精子 - 卵子相互作用中断诱导不育，还会破坏正常的卵巢功能。我们以狨猴（Callithrix jacchus）为动物模型，评估了诱导针对纯化重组人ZP3（rec.hZP3）和人ZP3肽的主动免疫的后果。虽然接受rec.hZP3的动物出现了不育，但这与卵巢功能障碍有关，其特征为卵泡生成受抑制和原始卵泡池耗竭。通过表位作图研究确定的连续人ZP3肽免疫并未诱导卵巢病变，但抗体滴度不足以显著且持续地抑制生育能力。显然，需要进一步研究以鉴定和分离透明带糖蛋白上能够诱导不育而无任何副作用的表位。
