Aitken R J, Paterson M, van Duin M
MRC Reproductive Biology Unit, Edinburgh, Scotland.
Am J Reprod Immunol. 1996 Mar;35(3):175-80. doi: 10.1111/j.1600-0897.1996.tb00027.x.
To investigate the contraceptive potential of the zona pellucida.
Generation of antibodies against native and recombinant zona glycoproteins which have then been assessed for their capacity to disrupt sperm-zona interaction in vivo and in vitro. The animal model selected for these studies was the common marmoset and the end points examined were antibody titre, ovarian cyclicity and fertility.
The fact that antibodies against the major zona glycoprotein, ZP3, block both the primary and secondary phases of sperm-zona interaction suggests that this molecule might have potential for contraceptive vaccine development. Active immunization of marmoset monkeys with native porcine ZP3 or recombinant human ZP3 produced long term infertility but also precipitated a premature decline in the primordial follicle population.
Future studies will have to determine whether a safe, effective vaccine can be engineered by coupling unique B-cell epitopes from ZP3 to foreign T-cell antigens.
研究透明带的避孕潜力。
制备针对天然和重组透明带糖蛋白的抗体，然后评估其在体内和体外破坏精子与透明带相互作用的能力。这些研究选用的动物模型是普通狨猴，检测的终点指标是抗体滴度、卵巢周期性和生育能力。
针对主要透明带糖蛋白ZP3的抗体能阻断精子与透明带相互作用的初级和次级阶段，这表明该分子可能具有开发避孕疫苗的潜力。用天然猪ZP3或重组人ZP3对狨猴进行主动免疫可导致长期不育，但也会使原始卵泡数量过早减少。
未来的研究必须确定能否通过将ZP3独特的B细胞表位与外来T细胞抗原偶联来设计出一种安全、有效的疫苗。
