Kiers A, Drost A P, van Soolingen D, Veen J
GGD, afd. Tuberculosebestrijding, Leeuwarden.
Ned Tijdschr Geneeskd. 1996 Nov 16;140(46):2290-3.
Description of the value of modern DNA analysis ('restriction fragment length polymorphism'; RFLP) as a method for mapping tuberculosis transmission.
Descriptive.
Social Health Service Noord-Friesland, the Netherlands.
A large-scale tuberculosis investigation was conducted in Harlingen in 1993 when a 2,5 year old patient with tuberculous meningitis was reported. Source tracing and contact tracing extended from Harlingen to the west of the Netherlands and even abroad. Modern DNA analysis (RFLP) was used to map the tuberculosis transmission.
A total of 6519 persons were screened and 276 infected people were identified, of whom 49 were suffering from active tuberculosis. RFLP analysis showed in 27 of them a 'Harlingen' type Mycobacterium tuberculosis DNA pattern identical to that of the index patient. The source patient was finally traced in England. By the end of 1994 3 more patients were found with the same DNA pattern; their relation with the source patient and the outbreak of tbc remained unrevealed.
The Harlingen outbreak was extensive and characterized by time-consuming source tracing. Modern DNA analysis proved to be a very useful instrument in identifying the source case.
描述现代DNA分析（“限制性片段长度多态性”；RFLP）作为一种绘制结核病传播图谱方法的价值。
描述性研究。
荷兰北弗里斯兰省社会卫生服务机构。
1993年，当报告一名2.5岁的结核性脑膜炎患者时，在哈灵根进行了一项大规模结核病调查。传染源追踪和接触者追踪范围从哈灵根扩展到荷兰西部甚至国外。采用现代DNA分析（RFLP）绘制结核病传播图谱。
共筛查6519人，确定276人感染，其中49人患有活动性结核病。RFLP分析显示，其中27人的结核分枝杆菌DNA模式为“哈灵根”型，与索引患者相同。传染源患者最终在英国被追踪到。到1994年底，又发现3例具有相同DNA模式的患者；他们与传染源患者的关系以及结核病的爆发情况仍未查明。
哈灵根疫情范围广泛，其特点是传染源追踪耗时。现代DNA分析被证明是识别传染源病例的非常有用的工具。
