Versée V, Barel A O
Biochem J. 1979 Jun 1;179(3):705-7. doi: 10.1042/bj1790705.
The binding of bilirubin to rat-alpha-foetoprotein has been measured by changes in the absorption spectrum and by the appearance of two intense circular-dichroism bands. Furthermore binding of this bile pigment has been demonstrated by a decrease of its enzymic oxidation rate and by competition experiments with bovine serum albumin-agarose. The binding parameters have been determined as follows: n = 1.0 mol bound/mol of protein and Ka = 2.9 X 10(6) M-1. Competition of oestradiol for the bilirubin-binding site has not been established.
已通过吸收光谱的变化以及两条强圆二色性带的出现来测定胆红素与大鼠α-甲胎蛋白的结合。此外，这种胆色素的结合还通过其酶促氧化速率的降低以及与牛血清白蛋白-琼脂糖的竞争实验得到证实。结合参数已确定如下：n = 1.0摩尔结合物/摩尔蛋白质，Ka = 2.9×10⁶ M⁻¹。尚未确定雌二醇对胆红素结合位点的竞争情况。
