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[苦荞粉与普通小麦粉的电泳分析]

[Electrophoretic analysis of buckwheat flour compared with regular wheat flour].

作者信息

de Francischi M L, Salgado J M, Carvalho M T, Derbyshire E

机构信息

Setor de Nutrição Humana e Alimentos, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Brasil.

出版信息

Arch Latinoam Nutr. 1994 Dec;44(4):274-6.

PMID:8984969
Abstract

The composition of the alcohol soluble proteins (prolamins) obtained from buckwheat meal and common wheat flour by two procedures were analysed by electrophoresis at pH 3.1 and, after dissociation, in the presence of sodium dodecyl sulphate at pH 8. The profiles obtained from the prolamin fraction of buckwheat were very different, qualitatively and quantitatively, from those of the prolamin of common wheat. It is probable therefore that the adverse effects associated with the presence of wheat gliadin in diets of patients with celiac disease would be reduced and possibly avoided if wheat flour were replaced by flour from buckwheat.

摘要

通过两种方法从荞麦粉和普通小麦粉中获得的醇溶蛋白(麦醇溶蛋白)的组成,在pH 3.1条件下进行电泳分析,并在解离后于pH 8的十二烷基硫酸钠存在下进行分析。从荞麦醇溶蛋白部分获得的图谱在质量和数量上与普通小麦醇溶蛋白的图谱有很大不同。因此,如果用荞麦粉代替小麦粉,乳糜泻患者饮食中与小麦麦醇溶蛋白存在相关的不良影响可能会减少甚至避免。

相似文献

1
[Electrophoretic analysis of buckwheat flour compared with regular wheat flour].[苦荞粉与普通小麦粉的电泳分析]
Arch Latinoam Nutr. 1994 Dec;44(4):274-6.
2
Enzyme-linked immunosorbent assay for quantitation of cereal proteins toxic in coeliac disease.用于定量检测乳糜泻中有毒谷物蛋白的酶联免疫吸附测定法。
Clin Chim Acta. 1988 Dec 30;178(3):261-70. doi: 10.1016/0009-8981(88)90234-3.
3
Chemical, nutritional and technological characteristics of buckwheat and non-prolamine buckwheat flours in comparison of wheat flour.与小麦粉相比,荞麦及非醇溶蛋白荞麦粉的化学、营养和工艺特性
Plant Foods Hum Nutr. 1994 Dec;46(4):323-9. doi: 10.1007/BF01088431.
4
Chromatographic and mass spectrometry analysis of wheat flour prolamins, the causative compounds of celiac disease.小麦醇溶蛋白的色谱和质谱分析,该物质是乳糜泻的致病化合物。
Food Funct. 2017 Aug 1;8(8):2712-2721. doi: 10.1039/c7fo00266a. Epub 2017 Jul 13.
5
Sequential extraction and quantitative recovery of gliadins, glutenins, and other proteins from small samples of wheat flour.从小麦粉小样本中连续提取并定量回收醇溶蛋白、谷蛋白及其他蛋白质。
J Agric Food Chem. 2005 Mar 9;53(5):1575-84. doi: 10.1021/jf048697l.
6
Extraction of up to 95% of wheat (Triticum aestivum) flour protein using warm sodium dodecyl sulfate (SDS) without reduction or sonication.在不进行还原或超声处理的情况下,使用温热的十二烷基硫酸钠(SDS)提取高达95%的小麦(普通小麦)面粉蛋白。
J Agric Food Chem. 2008 Aug 27;56(16):7431-8. doi: 10.1021/jf800776b. Epub 2008 Jul 11.
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The prolamin storage proteins of wheat and related cereals.小麦及相关谷物的醇溶谷蛋白贮藏蛋白。
Prog Biophys Mol Biol. 1994;61(1):37-59.
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Endogenous factors responsible for the textural characteristics of buckwheat products.导致荞麦制品质地特性的内源性因素。
J Nutr Sci Vitaminol (Tokyo). 1997 Feb;43(1):101-11. doi: 10.3177/jnsv.43.101.
9
A radioimmunoassay for wheat gliadin to assess the suitability of gluten free foods for patients with coeliac disease.一种用于检测小麦醇溶蛋白的放射免疫分析法,以评估无麸质食品对乳糜泻患者的适用性。
Clin Exp Immunol. 1985 Mar;59(3):703-8.
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Compared use of HPLC and FZCE for cluster analysis of Triticum spp and for the identification of T. durum adulteration.比较高效液相色谱法(HPLC)和毛细管区带电泳法(FZCE)在小麦属聚类分析及硬粒小麦掺假鉴定中的应用。
J Agric Food Chem. 2004 Jun 30;52(13):4080-9. doi: 10.1021/jf034881f.

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[Anaphylactic shock due to French galette. Type I allergic reaction to buckwheat].[法式可丽饼引发的过敏性休克。对荞麦的I型过敏反应]
Hautarzt. 2005 Feb;56(2):160-3. doi: 10.1007/s00105-004-0746-z.