来自噬菌体展示文库的生物合成脂质修饰的人单链抗体片段作为免疫脂质体的靶向分子。
Biosynthetically lipid-modified human scFv fragments from phage display libraries as targeting molecules for immunoliposomes.
作者信息
de Kruif J, Storm G, van Bloois L, Logtenberg T
机构信息
Department of Immunology, Utrecht University, The Netherlands.
出版信息
FEBS Lett. 1996 Dec 16;399(3):232-6. doi: 10.1016/s0014-5793(96)01335-x.
A human anti-CD22 single chain (sc) Fv antibody fragment from a synthetic phage antibody display library was biosynthetically lipid-tagged by using Escherichia coli lipoprotein sequences. The purified anti-CD22 scFv lipoprotein was incorporated into liposomes by detergent dilution. Anti-CD22 immunoliposomes were shown to bind specifically in a dose- and time-dependent manner to CD22+ cell lines and CD22+ B-lymphocytes present in freshly isolated samples of blood mononuclear cells. The immunoliposomes were demonstrated to accumulate in intracellular compartments. Biosynthetically lipid-tagged human scFv antibody fragments isolated from phage display libraries may facilitate the construction of immunoliposomes with improved properties.
利用大肠杆菌脂蛋白序列对来自合成噬菌体抗体展示文库的人抗CD22单链（sc）Fv抗体片段进行生物合成脂质标记。通过去污剂稀释将纯化的抗CD22 scFv脂蛋白掺入脂质体中。结果表明，抗CD22免疫脂质体以剂量和时间依赖性方式特异性结合于新鲜分离的血液单核细胞样品中存在的CD22+细胞系和CD22+B淋巴细胞。已证实免疫脂质体在细胞内区室中积累。从噬菌体展示文库中分离的生物合成脂质标记的人scFv抗体片段可能有助于构建具有更好性能的免疫脂质体。
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