Allander T, Beyene A, Jacobson S H, Grillner L, Persson M A
Karolinska Institute, Department of Medicine, Karolinska Hospital, Stockholm, Sweden.
J Infect Dis. 1997 Jan;175(1):26-31. doi: 10.1093/infdis/175.1.26.
The antibody response to the hypervariable region of the E2 protein (HVR1) of hepatitis C virus (HCV) was studied in 5 patients who were infected by a common virus strain during an outbreak in a hemodialysis unit. Two patients resolved the infection, while 3 developed chronic HCV infection. For studying the antibody response to HVR1 during the early phase of infection, a Western blot assay using recombinant phage displaying HVR1 was developed. The 2 patients with resolving infection had a more rapid antibody response to HVR1 than did the patients developing chronic infection. Anti-HVR1 antibodies were repeatedly absent in 1 of the chronically infected patients. Antibodies to recombinant E2 protein occurred later than the anti-HVR1 antibodies and did not correlate with resolution of the infection. Thus, the present results suggest that early appearance of antibodies to the HVR1 may predict clearance of HCV infection.
在一家血液透析单位爆发的一起疫情中，对5名感染了同一常见病毒株的患者进行了丙型肝炎病毒（HCV）E2蛋白高变区（HVR1）抗体反应的研究。2名患者感染得以清除，而3名患者发展为慢性HCV感染。为了研究感染早期对HVR1的抗体反应，开发了一种使用展示HVR1的重组噬菌体的蛋白质印迹分析方法。与发展为慢性感染的患者相比，2名感染得以清除的患者对HVR1的抗体反应更快。1名慢性感染患者的抗HVR1抗体反复缺失。重组E2蛋白抗体出现的时间晚于抗HVR1抗体，且与感染的清除无关。因此，目前的结果表明，抗HVR1抗体的早期出现可能预示着HCV感染的清除。
