Del Río J A, Heimrich B, Borrell V, Förster E, Drakew A, Alcántara S, Nakajima K, Miyata T, Ogawa M, Mikoshiba K, Derer P, Frotscher M, Soriano E
Department of Animal and Plant Cell Biology, Faculty of Biology, University of Barcelona, Spain.
Nature. 1997 Jan 2;385(6611):70-4. doi: 10.1038/385070a0.
During development of the nervous system, specific recognition molecules provide the cues necessary for the formation of neural connections. In some regions, guiding cues for axonal pathfinding and target selection are provided by specific cells that exist only transiently during development, such as the floorplate or the cortical subplate. In the hippocampus, distinct groups of fibres innervate different layers. We have tested the hypothesis that transient neurons in the hippocampus provide positional information for the targeting of these fibres. Here we report that ablation of Cajal-Retzius cells in organotypic slice cultures of hippocampus prevented the ingrowth of entorhinal but not of commissural afferents. Experiments inhibiting Reelin (an extracellular matrix protein expressed by Cajal-Retzius cells) and analysis of reeler mutant mice showed dramatic abnormalities in the development of entorhinal afferents. Thus Cajal-Retzius cells and reelin are essential for the formation of layer-specific hippocampal connections.
在神经系统发育过程中，特定的识别分子为神经连接的形成提供了必要的线索。在某些区域，轴突寻路和靶标选择的引导线索由发育过程中仅短暂存在的特定细胞提供，如底板或皮质下板。在海马体中，不同的纤维群支配不同的层。我们已经检验了这样一个假说，即海马体中的短暂神经元为这些纤维的靶向提供位置信息。在此我们报告，在海马体器官型切片培养物中去除 Cajal-Retzius 细胞可阻止内嗅传入纤维的长入，但不影响连合传入纤维。抑制 Reelin（一种由 Cajal-Retzius 细胞表达的细胞外基质蛋白）的实验以及对 reeler 突变小鼠的分析表明，内嗅传入纤维的发育存在显著异常。因此，Cajal-Retzius 细胞和 Reelin 对于层特异性海马体连接的形成至关重要。
