通过R18荧光和PCR检测监测牛白血病病毒与靶细胞的融合。
Fusion of bovine leukemia virus with target cells monitored by R18 fluorescence and PCR assays.
作者信息
Zarkik S, Defrise-Quertain F, Portetelle D, Burny A, Ruysschaert J M
机构信息
Laboratoire de Chimie-Physique des Macromolécules aux Interfaces, Université Libre de Bruxelles, Brussels, Belgium.
出版信息
J Virol. 1997 Jan;71(1):738-40. doi: 10.1128/JVI.71.1.738-740.1997.
Abstract
PCR and R18 fluorescence dequenching assays have been combined to monitor the kinetics of fusion of bovine leukemia virus with target cells (CC81, OVK, or Raji). Antibodies raised against gp51 allow us to demonstrate that not only the hydrophobic N-terminal domain of the transmembrane glycoprotein gp30 but also specific domains of gp51 (amino acids 39 to 103) are involved in bovine leukemia virus-cell fusion.
摘要
聚合酶链反应（PCR）和R18荧光猝灭分析已被结合起来，用于监测牛白血病病毒与靶细胞（CC81、OVK或Raji）融合的动力学过程。针对gp51产生的抗体使我们能够证明，不仅跨膜糖蛋白gp30的疏水N端结构域，而且gp51的特定结构域（氨基酸39至103）都参与了牛白血病病毒与细胞的融合。
相似文献
1
Fusion of bovine leukemia virus with target cells monitored by R18 fluorescence and PCR assays.通过R18荧光和PCR检测监测牛白血病病毒与靶细胞的融合。
J Virol. 1997 Jan;71(1):738-40. doi: 10.1128/JVI.71.1.738-740.1997.
2
Synthesis of bovine leukemia virus antigens in Escherichia coli.
Arch Exp Veterinarmed. 1990;44(6):909-16.
3
A sensitive luminescence syncytium induction assay (LuSIA) based on a reporter plasmid containing a mutation in the glucocorticoid response element in the long terminal repeat U3 region of bovine leukemia virus.基于含有牛白血病病毒长末端重复 U3 区糖皮质激素反应元件突变的报告质粒的敏感发光合胞体诱导测定（LuSIA）。
Virol J. 2019 May 20;16(1):66. doi: 10.1186/s12985-019-1172-2.
4
Evaluation of viral membrane fusion assays. Comparison of the octadecylrhodamine dequenching assay with the pyrene excimer assay.病毒膜融合测定的评估。十八烷基罗丹明去淬灭测定法与芘激基缔合物测定法的比较。
Biochemistry. 1993 Oct 26;32(42):11330-7. doi: 10.1021/bi00093a009.
5
Bovine leukaemia proviral DNA detection in cattle using the polymerase chain reaction.利用聚合酶链反应检测牛的牛白血病前病毒DNA
Vet Microbiol. 1990 Nov;25(2-3):117-29. doi: 10.1016/0378-1135(90)90071-3.
6
7
Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1.多克隆牛血清而非病毒中和单克隆抗体可阻断牛白血病病毒（BLV）糖蛋白51（gp51）与重组牛白血病病毒受体BLVRcp1的结合。
J Virol. 1997 Apr;71(4):3263-7. doi: 10.1128/JVI.71.4.3263-3267.1997.
8
Molecular characterization of bovine leukemia virus from Moldovan dairy cattle.摩尔多瓦奶牛中牛白血病病毒的分子特征
Arch Virol. 2017 Jun;162(6):1563-1576. doi: 10.1007/s00705-017-3241-4. Epub 2017 Feb 17.
9
Detection of bovine leukemia virus RNA in serum using the polymerase chain reaction.
J Virol Methods. 1993 Jan;41(1):101-12. doi: 10.1016/0166-0934(93)90166-o.
10
Bovine leukaemia virus: rapid detection of proviral DNA by nested PCR in blood and organs of experimentally infected calves.牛白血病病毒：通过巢式PCR在实验感染小牛的血液和器官中快速检测前病毒DNA
Vet Microbiol. 1994 Nov;42(2-3):191-204. doi: 10.1016/0378-1135(94)90018-3.
引用本文的文献
1
The effectiveness of colostral antibodies for preventing bovine leukemia virus (BLV) infection in vitro.初乳抗体在体外预防牛白血病病毒（BLV）感染的有效性。
BMC Vet Res. 2018 Dec 29;14(1):419. doi: 10.1186/s12917-018-1724-5.
2
Determinants of the Bovine Leukemia Virus Envelope Glycoproteins Involved in Infectivity, Replication and Pathogenesis.牛白血病病毒包膜糖蛋白在感染性、复制和发病机制中的决定因素
Viruses. 2016 Mar 24;8(4):88. doi: 10.3390/v8040088.
3
The many mechanisms of viral membrane fusion proteins.病毒膜融合蛋白的多种机制。
Curr Top Microbiol Immunol. 2005;285:25-66. doi: 10.1007/3-540-26764-6_2.
4
A point mutation in the binding subunit of a retroviral envelope protein arrests virus entry at hemifusion.逆转录病毒包膜蛋白结合亚基中的一个点突变使病毒进入在半融合阶段停止。
J Virol. 2004 Jan;78(1):473-81. doi: 10.1128/jvi.78.1.473-481.2004.
本文引用的文献
1
THE CYCLE OF MULTIPLICATION OF VACCINIA VIRUS IN EARLE'S STRAIN L CELLS. I. UPTAKE AND PENETRATION.痘苗病毒在厄尔氏L细胞系中的增殖周期。I. 摄取与穿透
Virology. 1964 Nov;24:278-94. doi: 10.1016/0042-6822(64)90167-9.
2
Inactivation of PR8 influenza virus through the octadecylrhodamine B chloride membrane marker.通过氯化十八烷基罗丹明B膜标记物使PR8流感病毒失活。
Biochemistry. 1993 Jan 26;32(3):900-7. doi: 10.1021/bi00054a022.
3
Bovine leukemia virus, an animal model for the study of intrastrain variability.牛白血病病毒，一种用于研究菌株内变异性的动物模型。
J Virol. 1993 Feb;67(2):1086-9. doi: 10.1128/JVI.67.2.1086-1089.1993.
4
Identification of functional sites on bovine leukemia virus envelope glycoproteins using structural and immunological data.利用结构和免疫学数据鉴定牛白血病病毒包膜糖蛋白上的功能位点
Eur J Biochem. 1994 Jun 1;222(2):405-14. doi: 10.1111/j.1432-1033.1994.tb18879.x.
5
Differential effects of a hydrophobic tripeptide on human immunodeficiency virus type 1 (HIV-1)-induced syncytium formation and viral infectivity.一种疏水性三肽对1型人类免疫缺陷病毒（HIV-1）诱导的合胞体形成和病毒感染性的不同作用。
Biochem Biophys Res Commun. 1995 Mar 8;208(1):75-81. doi: 10.1006/bbrc.1995.1307.
6
Membrane fusion proteins of enveloped animal viruses.包膜动物病毒的膜融合蛋白。
Q Rev Biophys. 1983 May;16(2):151-95. doi: 10.1017/s0033583500005072.
7
Topographical analysis by monoclonal antibodies of BLV-gp51 epitopes involved in viral functions.利用单克隆抗体对参与病毒功能的BLV-gp51表位进行的拓扑分析。
Virology. 1982 Oct 30;122(2):353-62. doi: 10.1016/0042-6822(82)90235-5.
8
Monoclonal antibodies define eight independent antigenic regions on the bovine leukemia virus (BLV) envelope glycoprotein gp51.单克隆抗体可确定牛白血病病毒（BLV）包膜糖蛋白gp51上的八个独立抗原区域。
Virology. 1982 Oct 30;122(2):342-52. doi: 10.1016/0042-6822(82)90234-3.
9
Fluorescence method for measuring the kinetics of fusion between biological membranes.用于测量生物膜之间融合动力学的荧光方法。
Biochemistry. 1984 Nov 20;23(24):5675-81. doi: 10.1021/bi00319a002.
10
Functional regions of the envelope glycoprotein of human immunodeficiency virus type 1.1型人类免疫缺陷病毒包膜糖蛋白的功能区
Science. 1987 Sep 11;237(4820):1351-5. doi: 10.1126/science.3629244.
Zotero 插件