Steinbrecht R A
Max-Planck-Institut für Verhaltensphysiologie, Seewiesen, Germany.
Chem Senses. 1996 Dec;21(6):719-27. doi: 10.1093/chemse/21.6.719.
Pheromone-sensitive sensilla trichodea of nine moth species belonging to six families and three superfamilies of Lepidoptera were immunolabelled with an antiserum against the pheromone-binding protein of Antheraea polyphemus. Strong immunolabelling of the sensillum lymph was observed in all long sensilla trichodea of A. polyphemus, A. pernyi (Saturniidae), Bombyx mori (Bombycidae) and Manduca sexta (Sphingidae). Very weak labelling was found with all sensilla trichodea of Dendrolimus kikuchii (Lasiocampidae) and Lymantria dispar (Lymantriidae). In three noctuid species, some long sensilla trichodea were labelled strongly, some only weakly and some were not labelled at all. The fraction of long sensilla trichodea that were strongly labelled was large in Helicoverpa armigera, but small in Spodoptera littoralis and Autographa gamma. The observed cross-reactivity was not correlated with taxonomic relatedness of the species but rather with chemical relatedness of the pheromones used by these species, as a high labelling density was consistently observed in sensilla tuned to pheromones with an alcyl chain of 16 carbon atoms. The highly divergent specificity of pheromone-receptor cells in Noctuidae appears to be mirrored by a similar diversity of the pheromone-binding proteins in the sensilla trichodea. These data support the notion that pheromone-binding proteins participate in odorant discrimination.
用针对多音大蚕蛾信息素结合蛋白的抗血清对鳞翅目六个科、三个总科的九种蛾类的信息素敏感毛形感器进行免疫标记。在多音大蚕蛾、柞蚕（大蚕蛾科）、家蚕（蚕蛾科）和烟草天蛾（天蛾科）的所有长毛形感器中均观察到感器淋巴的强免疫标记。在日本松毛虫（枯叶蛾科）和舞毒蛾（毒蛾科）的所有毛形感器中发现标记非常弱。在三种夜蛾科物种中，一些长毛形感器被强烈标记，一些仅被弱标记，还有一些根本没有被标记。在棉铃虫中被强烈标记的长毛形感器比例很大，但在草地贪夜蛾和苜蓿银纹夜蛾中比例很小。观察到的交叉反应性与物种的分类相关性无关，而是与这些物种使用的信息素的化学相关性有关，因为在调谐到具有16个碳原子烷基链信息素的感器中始终观察到高标记密度。夜蛾科中信息素受体细胞高度不同的特异性似乎反映在毛形感器中信息素结合蛋白的类似多样性上。这些数据支持信息素结合蛋白参与气味辨别这一观点。
