Burkhardt M, Poković K, Gnos M, Schmid T, Kuster N
Swiss Federal Institute of Technology, Zurich, Switzerland.
Bioelectromagnetics. 1996;17(6):483-93. doi: 10.1002/(SICI)1521-186X(1996)17:6<483::AID-BEM8>3.0.CO;2-#.
Crawford TEM cells are often used to expose cell cultures or small animals in order to study the effects caused by high-frequency fields. They are self-contained, easy-to-use setups that provide a rather homogeneous field distribution in a large area around its center, corresponding approximately to far-field conditions. However, a number of conditions must be met if such TEM cells are intended to be used for in vitro experiments. For instance, poor interaction with the incident field must be maintained to avoid significant field disturbances in the TEM cell. This is best achieved with E-polarization, i.e., when the E-field vector is normal to the investigated cell layer lining the bottom of a synthetic Petri dish. In addition, E-polarization provides the most homogeneous field distribution of all polarizations within the entire layer of cells. In this paper, we present a detailed dosimetric assessment for 60 and 100 mm Petri dishes as well as for a 48-well titer plate at 835 MHz. The dosimetry was performed by using numerical computations. The modeling and the simplifications are validated by a second numerical technique and by experimental measurements. For thin liquid layers, an approximation formula is provided with which the induced field strength for many other experiments conducted in Petri dishes can be assessed reliably.
克劳福德横电磁室（TEM 室）常用于对细胞培养物或小动物进行辐照，以研究高频场产生的效应。它们是独立的、易于使用的装置，在其中心周围的大面积区域内提供相当均匀的场分布，大致对应于远场条件。然而，如果打算将此类 TEM 室用于体外实验，必须满足一些条件。例如，必须保持与入射场的相互作用较弱，以避免 TEM 室内出现明显的场干扰。这在 E 极化情况下最容易实现，即当电场矢量垂直于合成培养皿底部衬里的被研究细胞层时。此外，E 极化在整个细胞层内提供了所有极化中最均匀的场分布。在本文中，我们给出了 835 MHz 频率下 60 毫米和 100 毫米培养皿以及 48 孔滴定板的详细剂量学评估。剂量学是通过数值计算进行的。建模和简化通过第二种数值技术和实验测量进行了验证。对于薄液体层，提供了一个近似公式，利用该公式可以可靠地评估在培养皿中进行的许多其他实验的感应场强。
