Brazillet M P, Mignon-Godefroy K, Charreire J
INSERM U 283, Université René Descartes, Hôpital Cochin, Paris.
Exp Clin Endocrinol Diabetes. 1996;104 Suppl 3:23-5. doi: 10.1055/s-0029-1211675.
Experimental Autoimmune Thyroiditis (EAT) is characterized by autoreactive T and B cell responses, assessed by a marked lymphocytic infiltration of the thyroid gland by T cells and the occurrence of circulating autoantibodies (AAb) to thyroglobulin (Tg). It was recently reported that administration of denaturated exogenous antigens primes class I-restricted cytotoxic T cells in vivo. Since cytotoxic T cells are involved in EAT development, porcine Tg (pTg) was heat-denaturated, i.v. injected into CBA/J mice and features of EAT evaluated. Simultaneously, mice were immunized with pTg and adjuvants and evaluation of EAT performed. We found that heat-denaturated pTg (hdpTg) induced EAT in recipient mice similar to native pTg/adjuvants. Surprisingly, whereas Tg-specific cytotoxic T cells were regularly found in lymph node cells from hdpTg or native pTg immunized mice, proliferative responses were only detected using T cells from native pTg immunized mice. Autoantibodies to pTg were decreased by a factor 30 in sera from mice immunized with hdpTg. These data further emphasized the role of Tg-specific cytotoxic T cells in EAT.
实验性自身免疫性甲状腺炎（EAT）的特征是自身反应性T细胞和B细胞反应，通过T细胞对甲状腺的显著淋巴细胞浸润以及针对甲状腺球蛋白（Tg）的循环自身抗体（AAb）的出现来评估。最近有报道称，给予变性的外源性抗原可在体内引发I类限制性细胞毒性T细胞。由于细胞毒性T细胞参与EAT的发展，将猪Tg（pTg）进行热变性，静脉注射到CBA/J小鼠体内，并评估EAT的特征。同时，用pTg和佐剂免疫小鼠并进行EAT评估。我们发现热变性的pTg（hdpTg）在受体小鼠中诱导的EAT与天然pTg/佐剂相似。令人惊讶的是，虽然在hdpTg或天然pTg免疫小鼠的淋巴结细胞中经常发现Tg特异性细胞毒性T细胞，但仅使用天然pTg免疫小鼠的T细胞检测到增殖反应。用hdpTg免疫的小鼠血清中针对pTg的自身抗体减少了30倍。这些数据进一步强调了Tg特异性细胞毒性T细胞在EAT中的作用。
