Schnierle B S, Groner B
Institute for Experimental Cancer Research, Tumor Biology Center, Freiburg, Germany.
Gene Ther. 1996 Dec;3(12):1069-73.
The systemic delivery of genes will open new applications for gene therapy. The deployment of retroviral vectors for this purpose is being considered and requires the development of retroviral vectors with a defined target cell specificity of infection. Several reports have recently described attempts to engineer the envelope protein of murine retroviruses in order to expand the host range and enable them to infect specific human cells. The strategies are based on the introduction of binding sites specific for receptors on the surface of target cells. Although the attempts to manipulate the specificity of viral target cell recognition are ambitious and promising, they are not without pitfalls. We summarize the results obtained and the difficulties encountered.
基因的全身递送将为基因治疗开辟新的应用领域。为此正在考虑使用逆转录病毒载体，这需要开发具有明确感染靶细胞特异性的逆转录病毒载体。最近有几份报告描述了对鼠逆转录病毒包膜蛋白进行工程改造的尝试，以扩大宿主范围并使其能够感染特定的人类细胞。这些策略基于引入对靶细胞表面受体具有特异性的结合位点。尽管操纵病毒靶细胞识别特异性的尝试雄心勃勃且前景广阔，但并非没有问题。我们总结了所获得的结果和遇到的困难。
