Sicard R E, Mand W, Hwang E, Nguyen L
Department of Surgery, University of Minnesota, Minneapolis 55455, USA.
In Vivo. 1996 Nov-Dec;10(6):547-51.
Enhanced accumulation of granulation tissue in a wound healing model was observed in the presence of regenerating skeletal muscle. This study characterized the tissue accumulated in the normal wound healing and regeneration-adapted models. DNA, RNA, total protein, collagen, and noncollagen protein content, and protein:DNA and noncollagen protein:collagen ratios were determined using standard methods. DNA did not differ; but RNA increased and total protein decreased compared to early granulation tissue. Mean protein: DNA ratio was lower in regeneration-associated granulation tissue compared to age-matched normal and early granulation tissue. Collagen was reduced and noncollagen protein was increased in tissue surrounding regenerating muscle, yielding an elevated noncollagen:collagen ratio. The relative cellularity of granulation tissue was enhanced and collagen deposition potentially reduced, when accompanied by regeneration. Factors from a regeneration environment can interact with cells engaged in wound repair and potentially alter their behavior.
在存在再生骨骼肌的情况下，观察到伤口愈合模型中肉芽组织的积累增强。本研究对正常伤口愈合和适应再生模型中积累的组织进行了表征。使用标准方法测定DNA、RNA、总蛋白、胶原蛋白和非胶原蛋白含量，以及蛋白:DNA和非胶原蛋白:胶原蛋白比率。DNA没有差异；但与早期肉芽组织相比，RNA增加而总蛋白减少。与年龄匹配的正常和早期肉芽组织相比，再生相关肉芽组织中的平均蛋白:DNA比率较低。再生肌肉周围组织中的胶原蛋白减少，非胶原蛋白增加，导致非胶原蛋白:胶原蛋白比率升高。当伴有再生时，肉芽组织的相对细胞密度增加，胶原蛋白沉积可能减少。来自再生环境的因子可以与参与伤口修复的细胞相互作用，并可能改变它们的行为。
