Niikura H, Sasano H, Sato S, Yajima A
Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan.
Int J Gynecol Pathol. 1997 Jan;16(1):60-8. doi: 10.1097/00004347-199701000-00010.
The biologic significance of epidermal growth factor (EGF)-related proteins and EGF receptor (EGFR) in the development and progression of human ovarian carcinoma was studied in 7 ovarian cystadenomas, 6 mucinous tumors of low malignant potential (LMP), and 25 invasive adenocarcinomas by immunohistochemistry. Results were correlated with clinicopathologic features. We also examined immunoreactivity in five serous adenocarcinomas both before and after cisplatin chemotherapy. Amphiregulin (AR) expression was observed only in mucinous tumors (4 of 8 cystadenomas, 2 of 6 tumors of LMP, and 6 of 10 cystadenocarcinomas), but was not detected in the serous tumors or clear cell adenocarcinomas. EGF, cripto, and EGFR expression was significantly higher in mucinous cystadenocarcinomas than in mucinous cystadenomas or mucinous tumors of LMP. Three of five specimens obtained at a second operation after chemotherapy had more intense or diffuse immunostaining for transforming growth factor alpha (TGF-alpha) than the initial specimens did. Coexpression of more than two of the EGF-related proteins or EGFR significantly correlated with increased surgical stage in serous and clear cell carcinoma. AR expression seems to correlate with mucinous differentiation rather than with advanced stages of ovarian tumors. Our results indicate that expression of some EGF-related proteins is greater in certain subtypes of ovarian carcinomas than in their benign counterparts and that coexpression of these proteins is associated with advanced stage in serous and clear cell carcinoma. Increased TGF-alpha expression may also be related to ovarian tumor resistance to cisplatin chemotherapy.
通过免疫组织化学方法，在7例卵巢囊腺瘤、6例低恶性潜能（LMP）黏液性肿瘤和25例浸润性腺癌中研究了表皮生长因子（EGF）相关蛋白和EGF受体（EGFR）在人卵巢癌发生发展中的生物学意义。将结果与临床病理特征相关联。我们还检测了5例浆液性腺癌在顺铂化疗前后的免疫反应性。双调蛋白（AR）仅在黏液性肿瘤中表达（8例囊腺瘤中有4例、6例LMP肿瘤中有2例、10例黏液性囊腺癌中有6例），但在浆液性肿瘤或透明细胞腺癌中未检测到。EGF、cripto和EGFR在黏液性囊腺癌中的表达显著高于黏液性囊腺瘤或LMP黏液性肿瘤。化疗后二次手术获取的5个标本中有3个对转化生长因子α（TGF-α）的免疫染色比初始标本更强或更弥漫。在浆液性和透明细胞癌中，两种以上EGF相关蛋白或EGFR的共表达与手术分期增加显著相关。AR表达似乎与黏液性分化相关，而非与卵巢肿瘤的晚期阶段相关。我们的结果表明，某些EGF相关蛋白在某些卵巢癌亚型中的表达高于其良性对应物，并且这些蛋白的共表达与浆液性和透明细胞癌的晚期阶段相关。TGF-α表达增加也可能与卵巢肿瘤对顺铂化疗的耐药性有关。
