Wang Q P, Guan J L, Nakai Y
Department of Anatomy, Showa University School of Medicine, Tokyo, Japan.
Synapse. 1997 Jan;25(1):24-9. doi: 10.1002/(SICI)1098-2396(199701)25:1<24::AID-SYN3>3.0.CO;2-H.
A double immunocytochemical method combining the preembedding avidin biotin peroxidase complex technique and the postembedding immunogold technique was used to examine synaptic interactions between GABAergic and nitric oxide synthase containing neurons in the same tissue sections of the dorsal raphe nucleus of the Wistar white rat. Although a large number of immunogold stained GABAergic axon terminals were found to be presynaptic to dendrites containing nitric oxide synthase-like immunoreaction product, synapses between GABA-like immunoreactive axon terminals and nitric oxide synthase-like immunoreactive perikarya were rare. The labeled boutons were found to make symmetrical and asymmetrical synapses. No axo-axonic synapse was found. These results suggest that GABAergic neurons could modulate nitric oxide producing neurons in the dorsal raphe nucleus through direct synaptic relations.
采用预包埋抗生物素蛋白生物素过氧化物酶复合物技术和包埋后免疫金技术相结合的双重免疫细胞化学方法，在Wistar大白鼠中缝背核的同一组织切片中检测含γ-氨基丁酸（GABA）能神经元与含一氧化氮合酶神经元之间的突触相互作用。尽管发现大量免疫金染色的GABA能轴突终末位于含有一氧化氮合酶样免疫反应产物的树突的突触前，但GABA样免疫反应性轴突终末与一氧化氮合酶样免疫反应性胞体之间的突触很少见。标记的终扣形成对称和不对称突触。未发现轴-轴突触。这些结果表明，GABA能神经元可通过直接的突触联系调节中缝背核中产生一氧化氮的神经元。
