Melby P C, Valencia-Pacheco G, Andrade-Narváez F
Department of Medicine, University of Texas Health Science Center, San Antonio 78284-7881, USA.
Arch Med Res. 1996 Winter;27(4):473-9.
Immunity in leishmaniasis is mediated by T cells, but protective responses in humans have not been fully defined. In this study, the functional activity of CD4+ T cell clones derived from an immune individual was investigated to identify potentially protective responses. The T cells proliferated and produced interferon-gamma (IFN-gamma) in response to a soluble Leishmania donovani antigen extract and live amastigotes. There was considerable variation in the anti-leishmanial activity of the T cell clones when they were co-cultured with L. donovani infected monocytes isolated from an HLA-DR,DQ matched donor. All of the clones which demonstrated antigen specific reactivity by proliferation or cytokine production induced some degree of inhibition of intracellular parasite replication, but only a few of the clones induced pronounced leishmanicidal activity. There was strong correlation between the level of amastigote-induced IFN-gamma secretion and anti-leishmanial activity. This approach enables the identification of potentially protective immune responses in humans at the clonal level, and offers a means for the identification of the relevant antigen(s).
利什曼病的免疫由T细胞介导，但人类的保护性反应尚未完全明确。在本研究中，对源自一名免疫个体的CD4 + T细胞克隆的功能活性进行了研究，以确定潜在的保护性反应。T细胞对可溶性杜氏利什曼原虫抗原提取物和活无鞭毛体产生增殖反应并分泌γ干扰素（IFN-γ）。当这些T细胞克隆与从一名HLA-DR、DQ匹配供体分离的感染杜氏利什曼原虫的单核细胞共培养时，其抗利什曼原虫活性存在相当大的差异。所有通过增殖或细胞因子产生表现出抗原特异性反应的克隆均诱导了一定程度的细胞内寄生虫复制抑制，但只有少数克隆诱导了显著的杀利什曼原虫活性。无鞭毛体诱导的IFN-γ分泌水平与抗利什曼原虫活性之间存在很强的相关性。这种方法能够在克隆水平上鉴定人类潜在的保护性免疫反应，并为鉴定相关抗原提供了一种手段。
