Cumming R H, Robinson P M, Martin G F
School of Science and Technology, University of Teesside, Middlesbrough, Cleveland, UK.
Bioseparation. 1996 Feb;6(1):17-23.
Continuously grown cells from a glucose limited chemostat were flocculated with four different cationic polymers. The polymer was added to the cells either dropwise or as a slug at the start of the flocculation period. Dose curves for each polymer type and using each method of polymer addition were constructed. It was evident that classical overdose was possible with all four polymers if slug addition was used. Continuous addition produced dose curves overdose was possible with all four polymers if slug addition was used. Continuous addition produced dose curves with no overdosing except when low molecular weight, low charge density polymer was used. The dose curves could be combined if they were based on amount of charge added. The dose curve was not linear, but fitted a logarithmic model well. Charge density was much more important than MW of the polymer.
从葡萄糖限制恒化器中连续培养的细胞用四种不同的阳离子聚合物进行絮凝。在絮凝期开始时，将聚合物逐滴添加或作为一大团添加到细胞中。构建了每种聚合物类型以及使用每种聚合物添加方法的剂量曲线。很明显，如果使用一大团添加的方式，所有四种聚合物都可能出现经典的过量现象。连续添加除了使用低分子量、低电荷密度的聚合物时会产生过量外，其他情况下不会产生过量的剂量曲线。如果基于添加的电荷量，剂量曲线可以合并。剂量曲线不是线性的，但很好地拟合了对数模型。电荷密度比聚合物的分子量重要得多。
