苏云金芽孢杆菌135 kDa杀虫蛋白基因在酿酒酵母中的表达
Expression of 135-kDa insecticidal protein gene from Bacillus thuringiensis in the yeast Saccharomyces cerevisiae.
作者信息
Watanabe K, Toyoda M, Hashimoto H, Nakagawa K, Nakamura K, Yabusaki Y, Tanimura A, Saito Y
机构信息
Showa Woman's University, Tokyo, Japan.
出版信息
Biosci Biotechnol Biochem. 1996 Sep;60(9):1483-5. doi: 10.1271/bbb.60.1483.
Bacillus thuringiensis subsp. aizawai produces 130-kDa and 135-kDa (CryIA(a)) insecticidal proteins. When Saccharomyces cerevisiae was transformed by the vector carrying a cryIA(a) gene, the gene expression could not be observed. When the 5'-upstream region from the initiation codon was removed using a synthetic oligonucleotide, the CryIA(a) protein was successfully synthesized in yeast. The yeast extract containing CryIA(a) protein had insecticidal activity against Plutella xylostella larvae.
苏云金芽孢杆菌日本亚种产生130千道尔顿和135千道尔顿（CryIA(a)）杀虫蛋白。当酿酒酵母用携带cryIA(a)基因的载体转化时，未观察到基因表达。当使用合成寡核苷酸去除起始密码子上游的5'区域时，CryIA(a)蛋白在酵母中成功合成。含有CryIA(a)蛋白的酵母提取物对小菜蛾幼虫具有杀虫活性。
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