Gough S, Flynn O, Hack C J, Marchant R
Biotechnology Research Group, School of Applied Biological and Chemical Sciences, University of Ulster, Coleraine, Co. Londonberry, Northern Ireland.
Appl Microbiol Biotechnol. 1996 Sep;46(2):187-90. doi: 10.1007/s002530050803.
The use of molasses as a substrate for ethanol production by the thermotolerant yeast Kluyveromyces marxianus var. marxianus was investigated at 45 degrees C. A maximum ethanol concentration of 7.4% (v/v) was produced from unsupplemented molasses at a concentration of 23% (v/v). The effect on ethanol production of increasing the sucrose concentration in 23% (v/v) molasses was determined. Increased sucrose concentration had a similar detrimental effect on the final ethanol produced as the increase in molasses concentration. This indicated that the effect may be due to increased osmotic activity as opposed to other components in the molasses. The optimum concentration of the supplements nitrogen, magnesium, potassium and fatty acid for maximum ethanol production rate was determined using the Nelder and Mead (Computer J 7:308-313, 1965) simplex optimisation method. The optimum concentration of the supplements were 0.576 g1(-1) magnesium sulphate, 0.288 g1(-1) potassium dihydrogen phosphate and 0.36% (v/v) linseed oil. Added nitrogen in the form of ammonium sulphate did not affect the ethanol production rate.
在45摄氏度下，研究了用糖蜜作为耐热酵母马克斯克鲁维酵母（Kluyveromyces marxianus var. marxianus）生产乙醇的底物。在浓度为23%（v/v）的未添加其他成分的糖蜜中，最高乙醇浓度可达7.4%（v/v）。测定了在23%（v/v）糖蜜中增加蔗糖浓度对乙醇生产的影响。蔗糖浓度增加对最终乙醇产量的不利影响与糖蜜浓度增加的影响类似。这表明这种影响可能是由于渗透活性增加，而非糖蜜中的其他成分。使用Nelder和Mead（《计算机杂志》7：308 - 313，1965年）单纯形优化方法确定了用于最大乙醇生产率的氮、镁、钾和脂肪酸补充剂的最佳浓度。补充剂的最佳浓度分别为0.576 g1(-1) 硫酸镁、0.288 g1(-1) 磷酸二氢钾和0.36%（v/v）亚麻籽油。以硫酸铵形式添加的氮对乙醇生产率没有影响。
