重组甘氨酸-胰岛素样生长因子-II包涵体的离心回收与溶解：进料速率和再离心对蛋白质产量的影响。
Centrifugal recovery and dissolution of recombinant Gly-IGF-II inclusion-bodies: the impact of feedrate and re-centrifugation on protein yield.
作者信息
Wong H H, O'Neill B K, Middelberg A P
机构信息
Department of Chemical Engineering, University of Adelaide, Australia.
出版信息
Bioseparation. 1996 Jun;6(3):185-92.
The impact of centrifuge feedrate and multiple centrifuge passes on protein yield following recombinant Gly-Insulin-like Growth Factor II (Gly-IGF-II) inclusion-body dissolution has been investigated. Altering centrifuge feedrate did not significantly improve the overall protein yield following dissolution. Improved centrifuge recovery at a low feedrate was offset by poorer inclusion body paste purity. This reduced purity resulted in a significant loss of protein during inclusion-body dissolution due to proteolysis. Multiple centrifuge passes improved the inclusion-body paste purity. This resulted in a net improvement in the overall protein yield following dissolution. This work demonstrates that a strong interaction exists between centrifuge performance and inclusion-body dissolution for protease-sensitive products such as Gly-IGF-II.
研究了离心进料速度和多次离心对重组甘氨酸胰岛素样生长因子II（Gly-IGF-II）包涵体溶解后蛋白质产量的影响。改变离心进料速度并不能显著提高溶解后的总蛋白质产量。低进料速度下提高的离心回收率被较差的包涵体糊纯度所抵消。这种纯度降低导致在包涵体溶解过程中由于蛋白水解而造成蛋白质大量损失。多次离心提高了包涵体糊的纯度。这导致溶解后总蛋白质产量的净提高。这项工作表明，对于像Gly-IGF-II这样对蛋白酶敏感的产品，离心性能与包涵体溶解之间存在强烈的相互作用。
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