通过随机DNA扩增对新型隐球菌进行特征分析。
Characterization of Cryptococcus neoformans by random DNA amplification.
作者信息
Ruma P, Chen S C, Sorrell T C, Brownlee A G
机构信息
Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, NSW, Australia.
出版信息
Lett Appl Microbiol. 1996 Nov;23(5):312-6. doi: 10.1111/j.1472-765x.1996.tb00197.x.
Random amplified polymorphic DNA (RAPD) was optimized and used to distinguish between the varieties and serotypes of Cryptococcus neoformans. The RAPD technique distinguished between serotypes A, D or AD within C. neoformans var. neoformans, and revealed further differentiation within each serotype. Four RAPD profiles were clearly recognizable within C. neoformans var. gattii, although its two serotypes, B and C, were only differentiated with one primer combination out of seven.
对随机扩增多态性DNA（RAPD）技术进行了优化，并用于区分新型隐球菌的不同变种和血清型。RAPD技术能够区分新型隐球菌变种内的A、D或AD血清型，并揭示了每个血清型内的进一步分化。在加蒂隐球菌变种内可清晰识别出四种RAPD图谱，尽管其B和C两种血清型仅通过七种引物组合中的一种得以区分。
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